Physiological and Molecular Characteristics of Low-Temperature-Tolerant Indica Rice Variety Diantun 502 during the Seedling Stage
#Co-first author: m13988499100@163.com
Received date: 2024-08-05
Online published: 2025-05-14
This study selected the low-temperature-tolerant indica rice variety Diantun 502 and its near- allele low-temperatures sensitive line Diantun 506 as the research subjects, aiming to explore the differences in physiological metabolic activities and low-temperature response gene expression between these two rice varieties under low-temperature stress, thereby providing a theoretical basis for revealing the molecular mechanism of Diantun 502's low-temperature tolerance. The experiment subjected the seedlings of both rice varieties to low-temperature treatment under the same environmental conditions and systematically analyzed the changes in various physiological indicators in the leaves of rice seedlings under low-temperature stress, including water content, chlorophyll content, proline content, malondialdehyde content, soluble sugar content, and the activity of the antioxidant enzyme system. Additionally, this study also detected the changes in the expression levels of key genes related to rice low-temperature resistance, such as COLD1, OsRAN1, OsCAF1B, OsFAD8, OsAOX1a and OsPHY1. The results showed that Diantun 502 mainly respond to low temperature stress by increasing the content of proline, enhancing the activity of antioxidant enzymes, and upregulating the expression levels of genes such as OsCAF1B, OsFAD8, OsAOX1a, and OsPHY1, thereby protecting the rice seedlings from low-temperature damage.
SHI Yitong, YONG Yu, LI Qiuping, MA Wenqing, XU Xiaoyu, YUAN Xingqiao, BI Ning, BAI Xiaoqing, LEI Wenhong, WEN Jiancheng, LI Dandan . Physiological and Molecular Characteristics of Low-Temperature-Tolerant Indica Rice Variety Diantun 502 during the Seedling Stage[J]. China Rice, 2025 , 31(3) : 75 -80 . DOI: 10.3969/j.issn.1006-8082.2025.03.0012
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