Research Progress and Prospects of Doubled Haploid Technology in Rice
Received date: 2025-05-29
Online published: 2025-07-08
Doubled haploid(DH) technology accelerates the production of fully homozygous breeding lines within just 1 to 2 generations, representing a significant improvement over conventional methods that require 6 to 10 generations of selfing or backcrossing. The process involves four key steps: haploid induction, haploid identification, chromosome doubling, and the cultivation and application of doubled haploids(DHs). Haploid induction, which serves as the foundation of this system, currently employs two main approaches in rice: (1) the well-established anther culture method and (2) the emerging hybridization-based method utilizing haploid inducers (HIs). This review highlights recent advances in HI-mediated DH technology for rice, analyzes the technical challenges encountered at each step, and explores its future applications in rice breeding.
JIN Xingchen, HUANG Yuheng, XU Jiangmin, WANG Kejian, RAO Yuchun, LIU Chaolei . Research Progress and Prospects of Doubled Haploid Technology in Rice[J]. China Rice, 2025 , 31(4) : 32 -36 . DOI: 10.3969/j.issn.1006-8082.2025.04.007
| [1] | REN J J, WU P H, TRAMPE B, et al. Novel technologies in doubled haploid line development[J]. Plant Biotechnology Journal, 2017, 15(11): 1 361-1 370. |
| [2] | 胡建林, 周黎, 郑兴飞, 等. 水稻花药离体培养的研究现状与展望[J]. 农业科技通讯, 2019, 12(1): 57-61. |
| [3] | CHAIKAM V, MOLENAAR W, MELCHINGER A, et al. Doubled haploid technology for line development in maize: technical advances and prospects[J]. Theoretical and Applied Genetics, 2019, 132(12): 3 227-3 243. |
| [4] | KELLIHER T, STARR D, RICHBOURG L, et al. MATRILINEAL, a sperm-specific phospholipase, triggers maize haploid induction[J]. Nature, 2017, 542(7639): 105-109. |
| [5] | GILLES L M, KHALED A, LAFFAIRE J B, et al. Loss of pollen-specific phospholipase NOT LIKE DAD triggers gynogenesis in maize[J]. The EMBO Journal, 2017, 36(6), 707-717. |
| [6] | LIU C X, LI X, MENG D X, et al. A 4-bp insertion at ZmPLA1 encoding a putative phospholipase A generates haploid induction in maize[J]. Molecular Plant, 2017, 10(3): 520-522. |
| [7] | YAO L, ZHANG Y, LIU C X, et al. OsMATL mutation induces haploid seed formation in indica rice[J]. Nature Plants, 2018, 4(8): 530-533. |
| [8] | WANG C, LIU Q, SHEN Y, et al. Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes[J]. Nature Biotechnology, 2019, 37(3): 283-286. |
| [9] | LIU Z K, ZHONG Y, QI X L, et al. Haploids can be induced in knockout mutants of OsPLA1, but not OsDMP3 or OsDMP6, in rice[J]. The Crop Journal, 2024, 12(1): 213-221. |
| [10] | LIANG S Y, WEN Q, LU W Y, et al. The haploid induction ability analysis of various mutation of OsMATL and OsDMPs in rice[J]. BMC Biology, 2025, 23(1): 30. |
| [11] | LIU C L, YAN S, MAO F M, et al. Large-scale production of rice haploids by combining superior haploid inducer with PTGMS lines[J]. Plant Communications, 2024, 5(12): 101 067. |
| [12] | JANG J H, SEO H S, WIDIEZ T, et al. Loss-of-function of gynoecium-expressed phospholipase pPLAIIγ triggers maternal haploid induction in Arabidopsis[J]. The New Phytologist, 2023, 238(5): 1 813-1 824. |
| [13] | JANG J H, NOH G Y, SEO H S, et al. Loss of function of pollen-expressed phospholipase OsMATL2 triggers haploid induction in japonica rice[J]. Plant Physiology, 2023, 193(3): 1 749-1 752. |
| [14] | HU F Y, LIU C L, JIN X C, et al. OsPLDα2-dependent synthetic apomixis enables normal seed setting in hybrid rice via genome editing[J]. Science Bulletin, 2025. doi: 10.1016/j.scib.2025.05.022. |
| [15] | ZHANG X C, SHI C, LI S L, et al. A female in vivo haploid-induction system via mutagenesis of egg cell-specific peptidases[J]. Molecular Plant, 2023, 16(2): 471-480. |
| [16] | ZHONG Y, LIU C X, QI X L, et al. Mutation of ZmDMP enhances haploid induction in maize[J]. Nature Plants, 2019, 5(6): 575-580. |
| [17] | 胡风越, 王健, 王春, 等. 水稻DMP1-3基因突变体的创制及其单倍体诱导能力鉴定[J]. 中国水稻科学, 2025, 39(1): 55-66. |
| [18] | RAVI M, CHAN S W. Haploid plants produced by centromere-mediated genome elimination[J]. Nature, 2010, 464(7288): 615-618. |
| [19] | LV J, YU K, WEI J, et al. Generation of paternal haploids in wheat by genome editing of the centromeric histone CENH3[J]. Nature Biotechnology, 2020, 38(12): 1 397-1 401. |
| [20] | MAYAKADUWA R, SILVA T. Haploid induction in indica rice: exploring new opportunities[J]. Plants, 2023, 12(17): 3 118. |
| [21] | WANG J, CAO Y X, WANG K J, et al. Development of multiple-heading-date mtl haploid inducer lines in rice[J]. Agriculture, 2022, 12(6): 806. |
| [22] | WANG J, YAN H J, JIAO X Z, et al. Development of specific molecular and phenotypic marker-based haploid inducers in rice[J]. Agronomy, 2023, 13(6): 1 520. |
| [23] | CHEN C, XIAO Z J, ZHANG J W, et al. Development of in vivo haploid inducer lines for screening haploid immature embryos in maize[J]. Plants (Basel), 2020, 9(6): 739. |
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