Improvement of Blast Resistance and Rice Quality in the Sterile Rice Line 33S by Molecular Marker-assisted Selection
1st author: zhang383399789yi@126.com
Received date: 2025-09-20
Online published: 2026-03-11
33S is a thermo-sensitive genic male sterile (TGMS) line special for late hybrid rice, which known for its good combining ability, the series of hybrid combinations derived from 33S are widely popular in the market due to its short growth period, high yield potential, and excellent lodging resistance. However, its practical application in hybrid breeding is constrained by poor resistance to rice blast, both leaf blast and panicle blast reaching severity level 9, and a high amylose content of 24.9%. This study combined directional backcrossing and molecular marker-assisted selection to introduce the rice blast resistance gene Pigm and the amylopectin-regulating gene Wxb into 33S, successfully developing two improved lines, HS4 and HS5, harboring both target genes. Resistance evaluations and grain quality analyses revealed that the comprehensive blast resistance indices of HS4 and HS5 were 3.5 and 2.3, corresponding amylose contents were 11.7% and 11.6%, respectively. When the same paternal line was crossed with the improved strain and 33S, there were no significant differences in the main agronomic traits of the resulting combinations. Furthermore, whole-genome resequencing indicated that the genetic background recovery rates of HS4 and HS5 were 96.67% and 97.79%, respectively. The results revealed that pyramiding Pigm and Wxb significantly enhances both blast resistance and grain quality, achieving targeted improvement in both disease resistance and palatability of the 33S rice line.
ZHANG Yi, XIE Hongjun, ZENG Xiaoshan, XIAO Feng, TANG Guohua, ZHU Mingdong, YU Yinghong . Improvement of Blast Resistance and Rice Quality in the Sterile Rice Line 33S by Molecular Marker-assisted Selection[J]. China Rice, 2026 , 32(2) : 113 -117 . DOI: 10.3969/j.issn.1006-8082.2026.02.018
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