Using the thermo-sensitive genic male sterile (TGMS) donor Y58S and the restorer line Tefeng 2053 as parents, the two-line sterile line Xin 32061S was successfully developed through multi-generational selfing purification and directional selection. This sterile line is classified as a TGMS line, with a fertility transition critical temperature of approximately 23.5 ℃ and a stable sterile period lasting over 40 days. Xin 32061S exhibits excellent agronomic traits, including approximately 175 spikelets per panicle. It possesses well-developed stigma morphology, characterized by a surface area of 11.49 mm², a volume of 1.15 mm³, and a total exsertion rate of 68.5%. Furthermore, pollen round abortion is nearly absent, indicating high potential for outcrossing seed-setting. Genetic background analysis using gene chips revealed a high homozygosity rate of 99.43%. Genomic analysis indicated that Xin 32061S contains 14.42% japonica rice genetic components, representing a novel indica rice germplasm with a stable genetic background that integrates elite japonica genes. In addition to carrying the core TGMS gene tms5, this line aggregates multiple genes associated with superior traits. These include yield-related genes (Gn1a/OsCKX2, LSCHL4, GW8/OsSPL16), grain shape-related genes (GS3, OsCYP704A3), ideal plant architecture genes (D2/CYP90D2/SMG11, D61/OsBRI1), and resistance genes (LHCB5, Xa26/Xa3). Based on this sterile line, five hybrid rice combinations have been successfully developed. Among them, Xinliangyou 9328 and Xinliangyou 905 have passed the National Rice Variety Approval; meanwhile, Xinliangyou 6198, Xinliangyou 6112, and Xinliangyou Xiangzhan are currently undergoing regional and production trials in the Lower-Middle Yangtze River region and Henan Province, demonstrating broad application prospects.