UPLC-MS/MS法测定水稻中噁唑酰草胺和氰氟草酯及其代谢物的残留及膳食风险评估

doi:10.3969/j.issn.1006-8082.2021.02.012

中国稻米 ›› 2021, Vol. 27 ›› Issue (2): 57-62.DOI: 10.3969/j.issn.1006-8082.2021.02.012

UPLC-MS/MS法测定水稻中噁唑酰草胺和氰氟草酯及其代谢物的残留及膳食风险评估

陈国峰(), 尤红梅, 滕瑶, 刘峰, 张晓波, 董见南, 廖辉^*()

黑龙江省农业科学院农产品质量安全研究所,哈尔滨 150086

收稿日期:2020-11-13 出版日期:2021-03-20 发布日期:2021-03-20
通讯作者: 廖辉
作者简介:
第一作者：hljjiance@163.com
基金资助:
食品安全社会共治信息技术研究与应用示范（2017YFC1601905）

Simultaneous Determination of Metamifop and Cyhalofop-methyl and Their Metabolites in Rice by UPLC-MS/MS and Dietary Risk Assessment

Guofeng CHEN(), Hongmei YOU, Yao TENG, Feng LIU, Xiaobo ZHANG, Jiannan DONG, Hui LIAO^*()

Safety and Quality Institute of Agricultural Products, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

Received:2020-11-13 Online:2021-03-20 Published:2021-03-20
Contact: Hui LIAO
About author:
1st author: hljjiance@163.com

摘要/Abstract

摘要：

建立了使用分散固相萃取的QuEChERS前处理方法,并通过超高效液相色谱串联质谱（Ultra Performance Liquid Chromatography Tandem Mass Spectrometry,UPLC-MS/MS）对糙米、稻壳和秸秆样品中的噁唑酰草胺和氰氟草酯及其代谢物成功进行了定量测定。结果表明,水稻于2叶1心期施药1次,收获后测定稻谷和糙米中噁唑酰草胺、氰氟草酯及其代谢产物的残留量,均未被检出。通过将国家估算的每日摄入量与可接受的每日摄入量进行比较,评估了这些农药的风险系数（Risk Quotients,RQ）。膳食风险评估的结果显示,在糙米中的噁唑酰草胺和氰氟草酯的RQ分别为0.87%和1.90%,表明在GAP条件下施用噁唑酰草胺和氰氟草酯对消费者的健康风险较低。

关键词: 噁唑酰草胺, 氰氟草酯, 代谢产物, 超高效液相色谱-串联质谱（UPLC-MS/MS）, 膳食风险评估

Abstract:

A QuEChERS pretreatment method using dispersive solid-phase extraction was established, and the residual amount of metamifop and cyhalofop-methyl and their metabolites in brown rice, rice husk and straw samples were determined by ultra performance liquid chromatography tandem mass spectrometry （UPLC-MS/MS）. The results showed that, none of the residues of metamifop and cyhalofop-methyl in rice grain and brown rice harvested at maturity were detected. The risk quotients （RQ） for these pesticides were assessed by comparing the country's estimated daily intake with the acceptable daily intake. The results of the dietary risk assessment showed that the RQ of metamifop and cyhalofop-methyl in brown rice were 0.87% and 1.9%, respectively. Consumers have lower health risk after consumption.

Key words: metamifop, cyhalofop-methyl, metabolites, ultra performance liquid chromatography tandem mass spectrometry （UPLC-MS/MS）, dietary risk assessment

中图分类号:

O657.63
S511

陈国峰, 尤红梅, 滕瑶, 刘峰, 张晓波, 董见南, 廖辉. UPLC-MS/MS法测定水稻中噁唑酰草胺和氰氟草酯及其代谢物的残留及膳食风险评估[J]. 中国稻米, 2021, 27(2): 57-62.

Guofeng CHEN, Hongmei YOU, Yao TENG, Feng LIU, Xiaobo ZHANG, Jiannan DONG, Hui LIAO. Simultaneous Determination of Metamifop and Cyhalofop-methyl and Their Metabolites in Rice by UPLC-MS/MS and Dietary Risk Assessment[J]. China Rice, 2021, 27(2): 57-62.

图/表 8

表1 试验地气候条件及土壤类型

表2 噁唑酰草胺和氰氟草酯及其代谢物的质谱参数

图1 优化噁唑酰草胺和氰氟草酯及其代谢产物提取溶剂

表3 提取溶液中净化剂PSA、C18和GCB的优化

表4 噁唑酰草胺和氰氟草酯及其代谢物的标准曲线和LOD

表5 噁唑酰草胺和氰氟草酯及其代谢物的添加回收率

表6 稻谷、糙米和秸秆中噁唑酰草胺和氰氟草酯的最终残留量

表7 糙米中噁唑酰草胺和氰氟草酯的膳食风险评估

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UPLC-MS/MS法测定水稻中噁唑酰草胺和氰氟草酯及其代谢物的残留及膳食风险评估

Simultaneous Determination of Metamifop and Cyhalofop-methyl and Their Metabolites in Rice by UPLC-MS/MS and Dietary Risk Assessment

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