中国稻米

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超级早稻中早39的稻瘟病抗性基因测定

  1. 中国水稻研究所/水稻生物学国家重点实验室,杭州 310006
  • 出版日期:2017-09-20 发布日期:2017-09-20
  • 通讯作者: 杨长登:yangchangdeng@126.com
  • 基金资助:
    中央级公益性科研院所基本科研业务费专项( 2014RG001-3)

Rice Blast Resistant Gene Detection in the Super Early Indica Rice Zhongzao 39

  • Online:2017-09-20 Published:2017-09-20

摘要: 超级早稻中早39在多年的浙江省联品和区试中持久高抗稻瘟病,为明确其抗性遗传基础,用12个稻瘟病抗性基因Pi-d2、Pi-d3、Pi36、Pi37、Pi5、Pib、Piz、Pik-h、Piz-t、Pi9、Pi-ta 和Pi64的分子标记,对中早39进行抗性基因检测。结果表明,12对分子标记在中早39均有目的扩增条带;因Piz、Piz-t和 Pi9为复等位基因,中早39中至少含有10个稻瘟病抗性基因。这一结果为中早39的稻瘟病抗性遗传利用奠定了基础。

关键词: 中早39, 稻瘟病, 抗性鉴定, 抗性基因, 基因检测

Abstract: The super rice variety Zhongzao 39 showed high and durable resistant to rice blast.Twelve pairs of molecular markers for the target resistant genes: Pi-d2, Pi-d3, Pi36, Pi37, Pi5, Pib, Piz, Pik-h, Piz-t, Pi9, Pi-ta and Pi64, were used to understand the resistant genetic mechanism of Zhongzao 39. The results indicated that all of the molecular markers yielded positive bands with expected size. As Piz, Piz-t and Pi9 were allelic to each other, there were ten rice blast resistant genes were identified in Zhongzao 39. All these results provided valuable information to the utilization of Zhongzao 39 in rice blast resistant breeding.

Key words: Zhongzao39, rice blast, resistant assessment, resistant gene, gene screening

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