专论与研究

9个广西常规香稻品种香味及香味基因变异类型分析

展开
  • 广西农业科学院 水稻研究所/广西水稻遗传育种重点实验室,南宁 530007

收稿日期: 2022-07-18

  网络出版日期: 2022-11-17

基金资助

国家现代农业产业技术体系广西创新团队项目(nycytxgxcxtd-2021-01-03);广西农业科学院科技发展基金稳定资助科研团队项目(桂农科2021YT029);广西重点研发计划项目(桂科AB21238007);中央引导地方科技发展专项资金项目(桂科ZY19183020);中央引导地方科技发展专项资金项目(桂科ZY21195034)

Analysis of Fragrance and Fragrance Gene Variation Types of 9 Conventional Fragrant Rice Varieties in Guangxi

Expand
  • Rice Research Institute, Guangxi Academy of Agricultural Sciences/ Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning 530007, China

Received date: 2022-07-18

  Online published: 2022-11-17

摘要

为加快广西香稻品种选育及新育成品种的推广应用,利用感官法和仪器测定法对9个广西常规香稻品种的香味表型进行鉴定,利用吸嗅蒸汽法对其香味类型进行评价,并应用目前已经开发的6个香味基因变异类型的功能标记对其香味基因型进行鉴定。结果表明,感官法和仪器测定法鉴定结果一致,9个供试品种均为香稻品种;除桂野丰为芋头香型,其他8个品种均为爆米花香型;除桂茉香1号外,其他8个香稻品种均为第7外显子缺失8 bp变异类型。桂茉香1号香味评分75分,2-乙酰-1-吡咯啉含量74.838 μg/kg,却不属于本研究检测的6种Badh2 基因变异类型,有待进一步鉴定和研究。

本文引用格式

刘广林, 李虎, 吴子帅, 罗群昌, 李秋雯, 朱其南, 陈传华 . 9个广西常规香稻品种香味及香味基因变异类型分析[J]. 中国稻米, 2022 , 28(6) : 54 -58 . DOI: 10.3969/j.issn.1006-8082.2022.06.011

Abstract

In order to speed up the breeding and the extension and application of fragrant rice varieties in Guangxi, the aroma phenotypes of nine fragrant rice varieties in Guangxi were identified by sensory and instrumental methods, the aroma types were evaluated by steam sniffer method, and the aroma genotypes were identified by using functional markers of six aroma gene variants developed so far. The results showed that the identification results of sensory and instrumental methods were consistent, and the nine tested varieties were all fragrant rice varieties. Except Guiyefeng, which is taro flavor type, the other eight varieties were popcorn flavor type. All tested varieties were the genetic variation type of 8bp deletion in exon 7 except Guimoxiang 1. Guimoxiang 1 had a fragrance score of 75 points and 2-AP content of 74.838 μg/kg, but did not belong to the six Badh2 gene variants detected in this study, needed for further identification and research.

参考文献

[1] 胡培松, 唐绍清, 魏兴华. 泰国香米事件及启示[J]. 中国稻米, 2006, 12(4):1-2.
[2] 王丰, 李金华, 柳武革, 等. 一种水稻香味基因功能标记的开发[J]. 中国水稻科学, 2008, 22(4):347-352.
[3] 彭波, 孙艳芳, 陈报阳, 等. 水稻香味基因及其在育种中的应用研究进展[J]. 植物学报, 2017, 52 (6):797-807.
[4] BRADBURY L M, FITZGERALD T L, HENRY R J, et al. The gene for fragrance in rice[J]. Plant Biotechnology Journal, 2005, 3(3): 363-370.
[5] SHAO G N, TANG A, TANG S Q, et al. A new deletion mutation of fragrant gene and the development of three molecular markers for fragrance in rice[J]. Plant Breeding, 2011, 130(2): 172-176.
[6] HE Q, PARK Y J. Discovery of a novel fragrant allele and development of functional markers for fragrance in rice[J]. Molecular Breeding, 2015, 35(11): 1-10.
[7] MYINT K M, ARIKIT S, WANCHANA S, et al. A PCR-based marker for a locus conferring the aroma in Myanmar rice(Oryza sativa L.)[J]. Theoretical & Applied Genetics, 2012, 125(5): 887-896.
[8] SHI W W, YANG Y, CHEN S H, et al. Discovery of a new fragrance allele and the development of functional markers for the breeding of fragrant rice varieties[J]. Molecular Breeding, 2008, 22(2): 185-192.
[9] OOTSUKA K, TAKAHASHI I, TANAKA K, et al. Genetic polymorphisms in Japanese fragrant landraces and novel fragrant allele domesticated in northern Japan[J]. Breed Science, 2014, 64(2): 115-124.
[10] 王军, 杨杰, 陈志德, 等. 水稻香米基因标记的开发与应用[J]. 分子植物育种, 2008, 6(6):1209-1 212.
[11] MURRAY M G, THOMPSON W F. Rapid isolation of high molecular weight plant DNA[J]. Nucleic Acids Research, 1980, 8(19): 4 321-4 326.
[12] 陈传华, 李虎, 刘广林, 等. 广西香稻育种现状及发展策略[J]. 中国稻米, 2017, 23(6):117-120.
[13] 闫影, 张丽霞, 胡泽军, 等. 利用分子标记辅助选育早熟优质香稻新品种‘沪早香软1 号’[J]. 分子植物育种, 2019, 17(22):7408-7 413.
[14] 闫影, 诸光明, 张丽霞, 等. 水稻香味基因分子标记的开发及应用[J]. 西北植物学报, 2015, 35(2):269-274.
[15] 罗世友, 胡兰香, 熊焕金, 等. 应用分子标记辅助选育香型优质稻九香粘[J]. 江西农业学报, 2020, 32(9):18-22.
[16] 郑景生, 江良荣, 黄荣裕, 等. 应用香型分子标记辅助选育优质香稻新品种佳福香占[J]. 福建稻麦科技, 2021, 39(2):7-9.
[17] 王天生, 陈惠清, 谢旺有, 等. 利用功能性分子标记辅助选育香型杂交水稻不育系[J]. 分子植物育种, 2020, 18(23):7801-7 807.
[18] 李虎, 刘广林, 吴子帅, 等. 利用分子标记辅助选育优质香稻新品种‘桂香 99’[J]. 分子植物育种, https://kns.cnki.net/kcms/detail/46.1068.S.20210325.1834.014.html.
[19] 曾宇, 夏秀忠, 农保选, 等. 广西特色香稻地方品种香味及其香味基因型的鉴定[J]. 南方农业学报, 2017, 48(9):1548-1 553.
[20] KOVACH M J, CALINGACION M N, FITZGERALD M A, et al. The origin and evolution of fragrance in rice (Oryza sativa L.)[J]. Proceedings of the National Academy of Sciences, 2009, 106(34): 4 444-4 449.
[21] 黄娟, 刘开强, 邓国富, 等. 水稻香味基因荧光分子标记开发及育种应用[J]. 植物生理学报, 2020, 56(5):1015-1 022.
文章导航

/

浙ICP备05004719号-16
公安备案号:33010302003356
版权所有 © 《中国稻米》编辑部
地址:浙江省杭州市富阳区水稻所路28号 邮编:311400 电话:0571-63370271, 63370368 E-mail:zgdm@163.com
本系统由北京玛格泰克科技发展有限公司设计开发