专论与研究

井冈霉素配施纳米硅肥对水稻光合特性、防御酶活性和纹枯病防控效果的影响

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  • 1福建农业职业技术学院福州 350007
    2福建农林大学 林学院福州 350007
第一联系人:

第一作者:zhaoshimin2832@126.com

收稿日期: 2025-08-31

  网络出版日期: 2026-01-13

基金资助

福建省科技厅引导性项目(2021N0002)

Effects of Combined Application of Jinggangmycin and Nano-Silicon Fertilzer on Photosynthetic Characteristics, Defense Enzyme Activities, and Control Efficacy Against Rice Sheath Blight

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  • 1Fujian Vocational College of Agriculture, Fuzhou 350007, China
    2College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350007, China
First author contact:

1st author: zhaoshimin2832@126.com

Received date: 2025-08-31

  Online published: 2026-01-13

摘要

发展病害绿色防控技术有利于水稻产业健康可持续发展。为明确井冈霉素与纳米硅肥配施对水稻纹枯病的防控效果及对水稻光合特性、防御酶活性的影响,以野香优669为参试品种开展试验,旨在筛选出最优的配施方案。结果表明,与CK(喷施等量无菌水)相比,井冈霉素配施纳米硅肥能够显著提升水稻的光合特性。其中,喷施5%井冈霉素A可湿性粉剂125 g+纳米硅肥700 mL处理的表现最好。该处理下,水稻总叶绿素含量达到4.69 mg/g,净光合速率(Pn)为14.90 μmol/(m2·s),气孔导度(Gs)为0.47 μmol/(m2·s),细胞间隙CO2浓度(Ci)为268.70 μmol/mol,蒸腾速率(Tr)为5.30 mmol/(m2·s);潜在光化学效率(Fv/Fo)、PSII最大光化学效率(Fv/Fm)、光化学淬灭系数(qP)和非光化学淬灭系数(NPQ)等叶绿素荧光参数均显著高于其他处理;超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)及苯丙氨酸解氨酶(PAL)等抗氧化防御酶活性在该处理中均最高,分别为513.3、875.1、117.7和47.9 U/(g·h)。同时,采用该处理方式的水稻病情指数为2.18,防效达76.20%,对水稻纹枯病的防控效果在各处理中最佳。综上所述,喷施5%井冈霉素 A 可湿性粉剂 125 g+纳米硅肥 700 mL的处理能够改善水稻的叶绿素含量、光合特性及叶绿素荧光参数,激发抗氧化防御酶活性,对水稻纹枯病具有良好的防控效果。

本文引用格式

郭锦全, 周友军 . 井冈霉素配施纳米硅肥对水稻光合特性、防御酶活性和纹枯病防控效果的影响[J]. 中国稻米, 2026 , 32(1) : 64 -69 . DOI: 10.3969/j.issn.1006-8082.2026.01.011

Abstract

The development of environmentally friendly disease prevention and control technology is conducive to the healthy and sustainable development of rice industry. To clarify the control efficacy of combined application of jinggangmycin and nano-silicon fertilizer against rice sheath blight, as well as its effects on photosynthetic characteristics and defense enzyme activities in rice, an experiment was conducted using the rice cultivar Yexiangyou 669 as the test material, aiming to screen for the optimal combined application scheme. The results showed that, compared to the control (CK, sprayed with an equal volume of sterile water), the combined application of jinggangmycin and nano-silicon fertilizer significantly enhanced the photosynthetic characteristics of rice. Among all treatments, the T3 treatment (spraying 5% validamycin A wettable powder 125 g+nano-silicon fertilizer 700 mL) demonstrated the best performance. Under this treatment condition, the total chlorophyII content, the net photosynthetic rate, stomatal conductance, intercellular CO2 concentration and transpiration rate of rice were 4.69 mg/g, 14.9 μmol/(m2·s), 0.47μmol/(m2·s), 268.7 μmol/mol and 5.3 mmol/(m2·s) respectively. Chlorophyll fluorescence parameters, including potential photochemical efficiency (Fv/Fo), maximum photochemical efficiency of PSII (Fv/Fm), photochemical quenching coefficient (qP), and non-photochemical quenching coefficient (NPQ), were significantly higher than those in other treatments. The activities of antioxidant defense enzymes, such as superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL), reached their highest values, with specific activities of 513.3, 875.1, 117.7, and 47.9 U/(g·h), respectively. Meanwhile, under this treatment condition, the disease index and control effect of rice were 2.18 and 76.2 %, respectively, and the prevention and control of rice sheath blight reached the best effect. In conclusion, the T3 treatment improved chlorophyll content, photosynthetic characteristics, and chlorophyll fluorescence parameters in rice, activated antioxidant defense enzymes, and demonstrated excellent control efficacy against rice sheath blight.

参考文献

[1] 马军韬, 张国民, 王永力, 等. 翻耕深度对水稻稻瘟病和纹枯病发生程度的影响[J]. 中国植保导刊, 2024, 44(11):51-54.
[2] LIU W, WANG G L. Plant innate immunity in rice: A defense against pathogen infection[J]. National Science Review, 2016, 3(3): 295-308.
[3] 董红刚, 耿跃, 陈凤, 等. 水稻纹枯病药剂防治关口前移的用药时间和种类试验[J]. 江苏农业科学, 2021, 49(23):125-128.
[4] 张小燕, 邓钦阳, 李冬霞, 等. 水稻秸秆还田方式对纹枯病发生的影响[J]. 广西植保, 2008, 21(3):11-13.
[5] BIAN C H, DUAN Y B, WANG J Y, et al. Validamycin a induces broad-spectrum resistance involving salicylic acid and jasmonic acid/ethylene signaling pathways[J]. Molecular Plant Microbe Interactions, 2020, 33(12): 1 424-1 437.
[6] 邵美红, 程楚, 柯汉云, 等. 阿维菌素与井冈霉素混配对水稻主要病虫害的防治效果[J]. 浙江大学学报(农业与生命科学版), 2024, 50(2):308-316.
[7] 卓富彦, 张熠玚, 郭永旺, 等. “十四五”期间我国水稻病虫害发生规律演变及绿色防控技术集成创新[J]. 中国稻米, 2025, 31(4):9-12.
[8] 宁东峰, 梁永超. 硅调节植物抗病性的机理:进展与展望[J]. 植物营养与肥料学报, 2014, 20(5):1 280-1 287.
[9] SUN W C, ZHANG J, FAN Q H, et al. Silicon-enhanced resistance to rice blast is attributed to silicon-mediated defence resistance and its role as physical barrier[J]. European Journal of Plant Pathology, 2010, 128(1): 39-49.
[10] CHEN W, YAO X Q, CAI K Z, et al. Silicon alleviates drought stress of rice plants by improving plant water status, photosynthesis and mineral nutrient absorption[J]. Biological Trace Element Research, 2011, 142(1): 67-76.
[11] 陈茜午, 温蕊, 张永虎. 叶面喷施硅肥对谷子生长发育及产量的影响[J]. 北方农业学报, 2020, 48(1): 56-60.
[12] BELANGER R R, BENHAMOU N, MENZIES J G. Cytological evidence of an active role of silicon in wheat resistance to powdery mildew(Blumeria graminis f.sp.tritici)[J]. Phytopathology, 2003, 93: 402-412.
[13] 刘伟, 徐礼英, 汤强, 等. 硅对油菜抗菌核病侵染及抗氧化酶活性的影响[J]. 淮阴师范学院学报(自然科学版), 2024, 23(1):35-40.
[14] 刘俊渤, 常海波, 马景勇, 等. 纳米SiO2对水稻稻瘟病的抗病效应及对水稻生长发育的影响[J]. 吉林农业大学学报, 2012, 34(2):157-161.
[15] 宋双, 付立东, 王宇, 等. 种子不同处理对水稻干尖线虫病危害的影响[J]. 北方水稻, 2011, 41(2):32-34.
[16] 孙德权, 陆新华, 胡玉林, 等. 纳米硅材料对植物生长发育影响的研究进展[J]. 热带作物学报, 2019, 40(11):2 300-2 311.
[17] 徐若涵, 杨再强, 申梦吟, 等. 苗期低温胁迫对“红颜”草莓叶绿素含量及冠层高光谱的影响[J]. 中国农业气象, 2022, 43(2):148-158.
[18] 杨克泽, 汪亮芳, 马金慧, 等. 硅与杀菌剂配施对玉米叶片代谢、产量及茎腐病防治效果的影响[J]. 玉米科学, 2024, 32(1):168-175.
[19] 李月灵, 金则新, 王强, 等. 不同生境华东野核桃光合生理特性及叶绿素荧光参数比较[J]. 浙江大学学报(理学版), 2013, 40(2):221-229.
[20] PALMER J W, DAVIES S B, SHAW P W, et al. Growth and fruit quality of ‘Braeburn’ apple (Malus domestica) trees as influenced by fungicide programmes suitable for organic production[J]. New Zealand Journal of Crop and Horticultural Science, 2003, 31(2): 169-177.
[21] 彭小琴, 惠竹梅, 张晖, 等. 24-表油菜素内酯对农药处理下葡萄叶片光合特性和抗逆性的影响[J]. 干旱地区农业研究, 2015, 33(3):130-138.
[22] 刘井兰, 于建飞, 印建莉, 等. 化学农药对植物生理生化影响的研究进展[J]. 农药, 2006, 45(8): 511-514.
[23] 邓欢欢, 莫小丽, 朱星辉, 等. 纳米硅材料对植物生长发育和环境响应的影响研究进展[J]. 浙江农业科学, 2024, 65(6):1 496-1 501.
[24] QADOS A, MOFTAH A. Influence of silicon and nano-silicon on germination, growth and yield of faba bean (Vicia faba L.) under salt stress conditions[J]. American Journal of Experimental Agriculture, 2015, 5(6): 509-524.
[25] 张国良, 戴其根, 霍中洋, 等. 外源硅对纹枯病菌(Rhizoctonia solani)侵染下水稻叶片光合功能的改善[J]. 生态学报, 2008, 28(10):4 881-4 890.
[26] 王余, 朱雯倩, 王娓敏, 等. 微囊藻毒素对水稻幼苗生长与叶绿素荧光的影响[J]. 环境科学学报, 2015, 35(2):602-607.
[27] CHEN H X, HUANG X Y, CHEN H, et al. Effect of silicon spraying on rice photosynthesis and antioxidant defense system on cadmium accumulation[J]. Scientific Reports, 2024, 14: 15 265.
[28] FAUTEUX F, RÉMUS-BOREL W, MENZIES J G, et al. Silicon and plant disease resistance against pathogenic fungi[J]. FEMS Microbiology Letters, 2005, 249(1): 1-6.
[29] 徐佳宁, 刘钢, 张利云, 等. 高温胁迫对不同番茄品种叶片抗氧化系统的影响[J]. 山东农业科学, 2016, 48(10):27-31.
[30] 汪玉洁, 陈日远, 刘厚诚, 等. 纳米材料在农业上的应用及其对植物生长和发育的影响[J]. 植物生理学报, 2017, 53(6):933-942.
[31] HUANG C P, QIN N N, SUN L, et al. Selenium improves physiological parameters and alleviates oxidative stress in strawberry seedlings under low-temperature stress[J]. International Journal of Molecular Sciences, 2018, 19(7): 1 913.
[32] 冯宇霞. 硅在水稻对核盘菌非寄主抗性中的作用及对油菜菌核病抗性的影响[D]. 重庆: 西南大学, 2020.
[33] 于广星, 宫殿凯, 代贵金, 等. 硅肥对水稻增产提质抗病虫的影响研究进展[J]. 中国稻米, 2019, 25(1): 21-22.
[34] 邓接楼, 王艾平, 何长水, 等. 硅肥对水稻生长发育及产量品质的影响[J]. 广东农业科学, 2011, 38(12):58-61.
[35] MONAHAN B J, VILLEN J, MARGUERAT S, et al. Fission yeast SWI/SNF and RSC complexes show compositional and functional differences from budding yeast[J]. Nature Structural & Molecular Biology, 2008, 15(8): 873-880.
[36] 黄建华, 何东兵, 陈宏州, 等. 井冈霉素与氟环唑防治水稻纹枯病的配方离体筛选及田间应用效果[J]. 江苏农业科学, 2020, 48(23):108-110.
[37] 张穗, 许文霞, 薛银根, 等. 郑州郊区水稻纹枯病菌对井冈霉素敏感性的初步研究[J]. 中国生物防治, 1995(4):171-173.
[38] 马娟, 高波, 李秀花, 等. 噻唑膦与硅肥联合使用对甘薯茎线虫病的防治效果[J]. 植物保护, 2024, 50(2):307-312.
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