Study on Rice Seed Germination and Seedling Emergence and Related Problems in Direct Seeding Production

doi:10.3969/j.issn.1006-8082.2023.06.010

Abstract

Abstract:

The seedling emergence process of rice includes the stages of seed imbibition, protrusion, germination and seedling establishment, involving physiological and biochemical activities such as seed cell activation and repair, material decomposition and metabolism, and energy metabolism, and its regulatory network is very complex. Based on a brief introduction to the process of seed germination and seedling emergence, and its physiological and biochemical activities, this paper analyzed the factors that affect the germination and emergence of seeds, and put forward the problems related to seed germination and emergence in rice production, including the problem of whole seedling of direct-seeding rice troubled when it encountered flooding and low temperature stress at seedling stage.

Key words: rice, seed germination, seedling emergence, whole seedling of direct-seeding rice

摘要：

水稻的出苗过程经过了种子的吸胀、萌动、发芽、出苗等阶段，涉及种子细胞活化修复、物质分解与代谢、能量代谢等生理生化活动，其调控网络十分复杂。本文在简要介绍种子萌发和出苗过程、生理生化活动的基础上，分析了影响种子萌发出苗的因素，提出了直播稻生产上与种子萌发和出苗相关的难题，即直播稻芽苗期遇到淹水和低温胁迫困扰直播稻的一次性全苗问题。

关键词: 水稻, 种子萌发, 出苗, 直播全苗

CLC Number:

S511.04

ZHENG Guangjie, TAO Yi, SHEN Xinglian, YE Chang, XU Yanan, CHU Guang, XU Chunmei, WANG Danying. Study on Rice Seed Germination and Seedling Emergence and Related Problems in Direct Seeding Production[J]. China Rice, 2023, 29(6): 49-55.

郑广杰, 陶怡, 沈兴连, 叶昌, 徐亚楠, 褚光, 徐春梅, 王丹英. 水稻种子萌发出苗研究及直播生产上相关难题[J]. 中国稻米, 2023, 29(6): 49-55.

References 77

[1]	ZHANG Y P, ZHU D F, XIONG H, et al. Development and transition of rice planting in China[J]. Agricultural Science & Technology, 2012, 13(6): 1 270-1 276.
[2]	罗锡文, 王在满, 曾山, 等. 水稻机械化直播技术研究进展[J]. 华南农业大学学报, 2019, 40(5):1-13.
[3]	赵正洪, 戴力, 黄见良, 等. 长江中游稻区水稻产业发展现状、问题与建议[J]. 中国水稻科学, 2019, 33(6):553-564.
[4]	戴亿政, 罗锡文, 张明华, 等. 气吹集排式水稻旱直播机关键部件设计与试验[J]. 农业工程学报, 2020, 36(10):1-8.
[5]	KOORNNEEF M, BENTSINK L, HILHORST H. Seed dormancy and germination[J]. Current Opinion in Plant Biology, 2002, 5(1): 33-36.
[6]	王建华, 张春庆. 种子生产学[M]. 北京: 高等教育出版社, 2006.
[7]	张红生, 胡晋. 种子学[M]. 北京: 科学出版社, 2015.
[8]	李晓炜. 种子萌发过程中呼吸关键酶调控物质能量代谢规律研究[D]. 兰州: 兰州大学, 2016.
[9]	胡晋. 种子生物学[M]. 北京: 高等教育出版社, 2006.
[10]	王忠, 顾蕴洁, 李卫芳, 等. 水稻糊粉层的形成及其在萌发过程中的变化[J]. 扬州大学学报(自然科学版), 1998, 1(1):19-24.
[11]	RRJZEK M, STEVENSON C E, SOUTHARD A M, et al. Chemical genetics and cereal starch metabolism: structural basis of the non-covalent and covalent inhibition of barley β-amylase[J]. Molecular BioSystems, 2011, 7(3): 718-730.
[12]	廖登群, 张洪亮, 李自超, 等. 水稻β-淀粉酶基因密码子使用特性分析[J]. 中国农业大学学报, 2009, 14(5):1-11.
[13]	顾振新, 陈志刚, 蒋振晖. 赤霉素处理对糙米发芽力及其主要成分变化的影响[J]. 南京农业大学学报, 2003, 26(1):74-77.
[14]	刘国琴, 张曼夫. 生物化学[M]. 2版. 北京: 中国农业大学出版社, 2011.
[15]	叶煜. 水稻己糖激酶HXK9基因敲除与水稻种子萌发相关的生理机制研究[D]. 杭州: 浙江大学, 2020.
[16]	徐恒恒, 黎妮, 刘树君, 等. 种子萌发及其调控的研究进展[J]. 作物学报, 2014, 40(7):1141-1 156.
[17]	SIMMONDS J A, SIMPSON G M. Increased participation of pentose phosphate pathway in response to afterripening and gibberellic acid treatment in caryopses of Avena fatua[J]. Canadian Journal of Botany, 1971, 49: 1 833-1 840.
[18]	NONOGAKI H, BASSEL G W, BEWLEY J D. Germination—still a mystery[J]. Plant Science, 2010, 179: 574-581.
[19]	胡晋, 沈建平, 阮松林. 应用物质效率测定水稻种子活力的研究[J]. 种子, 1992(4):58-59.
[20]	王忠. 植物生理学[M]. 2版. 北京: 中国农业出版社, 2010.
[21]	FENG F J, MEI H W, FAN P Q, et al. Dynamic transcriptome and phytohormone profiling along the time of light exposure in the mesocotyl of rice seedling[J]. Scientific Reports, 2017, 7(1): 11 961.
[22]	李雪菲. 茉莉素通过OsMYC2与赤霉素互作调控水稻中胚轴伸长生长[D]. 武汉: 华中农业大学, 2022.
[23]	宾金华, 黄胜琴, 何树春, 等. 茉莉酸甲酯对水稻种子萌发和贮藏物质降解的影响[J]. 植物学报, 2001, 43(6):578-585.
[24]	宋松泉, 刘军, 唐翠芳, 等. 生长素代谢与信号转导及其调控种子休眠与萌发的分子机制[J]. 科学通报, 2020, 65(34):3924-3 943.
[25]	WANG Y F, HOU Y X, QIU J H, et al. Abscisic acid promotes jasmonic acid biosynthesis via a ‘SAPK10-bZIP72-AOC’ pathway to synergistically inhibit seed germination in rice (Oryza sativa)[J]. New Phytologist, 2020, 228(4): 1 336-1 353.
[26]	熊敏. 油菜素内酯与赤霉素协同调控水稻种子萌发的分子机制研究[D]. 扬州: 扬州大学, 2021.
[27]	WEI T, HE Z L, TAN X Y, et al. An integrated RNA-Seq and network study reveals a complex regulation process of rice embryo during seed germination[J]. Biochemical and Biophysical Research Communications, 2015, 464(1): 176-181.
[28]	崔欢, 高巧丽, 罗立新, 等. 水稻萌发期激素信号转导和谷胱甘肽代谢转录分析[J]. 中国水稻科学, 2021, 35(6):554-564.
[29]	钱海丰, 赵晓娟, 赵心爱. α-淀粉酶基因表达的调控[J]. 西北农业学报, 2003, 12(4):87-90.
[30]	ZHU G H, YE N H, ZHANG J H, et al. Glucose-induced delay of seed germination in rice is mediated by the suppression of ABA catabolism rather than an enhancement of ABA biosynthesis[J]. Plant and Cell Physiology, 2009, 50(3): 644-651.
[31]	GILL S S, TUTEJA N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J]. Plant Physiology and Biochemistry, 2010, 48(12): 909-930.
[32]	QUAN L J, ZHANG B, SHI W W, et al. Hydrogen peroxide in plants:A versatile molecule of the reactive oxygen species network[J]. Journal of Integrative Plant Biology, 2008, 50(1): 2-18.
[33]	GOMES M P, GARCIA Q S. Reactive oxygen species and seed germination[J]. Biologia, 2013, 68(3): 351-357.
[34]	JEEVAN K S P, RAJENDRA P S, BANERJEE R, et al. Seed birth to death: Dual functions of reactive oxygen species in seed physiology[J]. Annals of Botany, 2015, 116(4): 663-668.
[35]	ISHIBASHI Y, TAWARATSUMIDA T, ZHENG S H, et al. NADPH oxidases act as key enzyme on germination and seedling growth in barley (Hordeum vulgare L.)[J]. Plant Production Science, 2010, 13(1): 45-52.
[36]	KRANNER I, ROACH T, BECKETT R P, et al. Extracellular production of reactive oxygen species during seed germination and early seedling growth in Pisum sativum[J]. Journal of Plant Physiology, 2010, 167(10): 805-811.
[37]	BAILLY C. Active oxygen species and antioxidants in seed biology[J]. Seed Science Research, 2004, 14(2): 93-107.
[38]	YE N H, JIA L G, ZHANG J H. ABA signal in rice under stress conditions[J]. Rice, 2012, 5(1): 1.
[39]	李金华, 王丰, 廖亦龙, 等. 水稻种子活力的生理生化及遗传研究[J]. 分子植物育种, 2009, 7(4):772-777.
[40]	张安鹏, 钱前, 高振宇. 水稻种子活力的研究进展[J]. 中国水稻科学, 2018, 32(3):296-303.
[41]	张红生, 程金平, 王健康, 等. 水稻种子活力相关基因鉴定及分子调控机制[J]. 南京农业大学学报, 2019, 42(2):191-200.
[42]	孙群, 王建华, 孙宝启. 种子活力的生理和遗传机理研究进展[J]. 中国农业科学, 2007, 40(1):48-53.
[43]	宋超. 水稻种子人工老化过程中抗氧化系统的变化[D]. 北京: 中国农业科学院, 2012.
[44]	李俊周, 李梦琪, 刘磊, 等. 水稻种子H₂O₂流速和种子活力的关系研究[J]. 华北农学报, 2017, 32(4):189-194.
[45]	高厚玉, 景立权, 陈龙, 等. 自由空气中CO₂浓度和温度增高对水稻种子活力的影响[J]. 中国水稻科学, 2016, 30(4):371-379.
[46]	杨翠, 田宏跃, 覃廷英, 等. 不同类型水稻种子活力的比较研究[J]. 种子, 2019, 38(11):12-15.
[47]	常志远. 浙江省常规粳稻品种的休眠性、耐贮藏性与种子活力的评价研究[D]. 金华: 浙江师范大学, 2020.
[48]	王云智, 韩才斌, 李龙先, 等. 水稻直播技术的现状和进展[J]. 农业与技术, 2018, 38(15):21-122.
[49]	姜心禄, 永波, 付明全, 等. 直播方式和播种方式对直播稻生长发育及产量形成的影响[J]. 中国稻米, 2018, 24(3):58-61.
[50]	杨文伟, 石亚飞, 高莲, 等. 播深和播量对宁夏地区直播稻幼苗素质和产量的影响[J]. 核农学报, 2023, 37(1):148-158.
[51]	杜萌, 马凌霄, 隋鑫, 等. 播种密度对滨海稻区水直播稻生长发育的影响[J]. 江苏农业科学, 2022, 50(14):61-67.
[52]	熊怀阳, 李阳生. 水稻的耐淹性状及其Sub1基因[J]. 遗传, 2010, 32(9):886-892.
[53]	熊怀阳, 阳菁, 安保光, 等. 水稻适应淹水胁迫的分子机理及品种改良[J]. 武汉大学学报(理学版), 2013, 59(1):17-23.
[54]	TURNER F T, CHEN C C, MCCAULEY G N. Morphological development of rice seedlings in water at controlled oxygen levels[J]. Agronomy Journal, 1981, 73(3): 566-570.
[55]	ISMAIL A M, ELLA E S, VERGARA G V, et al. Mechanisms associated with tolerance to flooding during germination and early seedling growth in rice (Oryza sativa)[J]. Annals of Botany, 2009, 103(2): 197-209.
[56]	MIRO B, ISMAIL A M. Tolerance of anaerobic conditions caused by flooding during germination and early growth in rice (Oryza sativa L)[J]. Frontiers in Plant Science, 2013, 4(1): 269.
[57]	HOFTTNANN0 B, KENDE H. On the role of abscisic acid and gibberellin in the regulation of growth in rice[J]. Plant Physiology, 1992, 99: 1 156-1 161.
[58]	KENDE H, DERKNAAP E, CHO H T. Deepwater rice: A model plant to study stem elongation[J]. Plant Physiology, 1998, 118: 1 105 -1 110.
[59]	HWANG Y S, THOMAS B R, RODRIGUEZ R L. Differential expression of rice α-Amylase genes during seedling development under anoxia[J]. Plant Molecular Biology, 1999, 40: 911-920.
[60]	GIBBS J, MORRELL S, VALDEZ A, et al. Regulation of alcoholic fermentation coleoptiles of two rice cultivars differing in tolerance to anoxia[J]. Journal of Experimental Botany, 2000, 51(345): 785-796.
[61]	KATO-NOGUCHI H, MOROKUMA M. Ethanolic fermentation and anoxia tolerance in four rice cultivars[J]. Journal of Plant Physiology, 2007, 164(2): 168-173.
[62]	NAKAZONO M, TSUJI H, LI Y H, et al. Expression of a gene encoding mitochondrial aldehyde dehydrogenase in rice increases under submerged conditions[J]. Plant Physiology, 2000, 124: 587-598.
[63]	LEE T K, LEE W S. Diauxic growth carbon in rice suspension cells grown on mixed sources of acetate and glucose[J]. Plant Physiology, 1996, 110: 465-470.
[64]	PAMPLONA R. Advanced lipoxidation end-products[J]. Chemico-Biological Interactions, 2011, 192: 14-20.
[65]	唐双勤,. 芽期低温、淹水胁迫对早籼稻生长特性与产量的影响[D]. 南昌: 江西农业大学, 2019.
[66]	李霞, 戴传超, 程睿, 等. 不同生育期水稻耐冷性的鉴定及耐冷性差异的生理机制[J]. 作物学报, 2006, 32(1):76-83.
[67]	曾韶西, 王以柔. 不同胁迫预处理提高水稻幼苗抗寒性期间膜保护系统的变化比较[J]. 植物学报, 1997, 39(4):308-314.
[68]	SHAW F J, LEE T M. Studies on the α-amylase from the germinated rice seeds[J]. Botanical Bulletin of Academia Sinica, 1984, 23: 41-46.
[69]	YU P H, JIANG N, FU W M, et al. ATP hydrolysis determines cold tolerance by regulating available energy for glutathione synthesis in rice seedling plants[J]. Rice, 2020, 13(1): 23.
[70]	郭确, 潘瑞炽. ABA对水稻幼苗抗冷性的影响[J]. 植物生理学报, 1984, 10(4):295-303.
[71]	全瑞兰, 扶定, 马汉云, 等. 水稻种子低温萌发的研究进展[J]. 中国农学通报, 2020, 36(29):7-14.
[72]	张建军, 刘红, 蒋小平, 等. 直播方式与播种量组合对水稻群体发育及产量构成的影响[J]. 中国农学通报, 2021, 37(5):1-7.
[73]	沈康荣, 李家军, 汪晓春, 等. 水稻覆膜直播栽培一播全苗技术研究[J]. 农业科技通讯, 2009(2):32-33
[74]	郑曼曼. 种子引发对旱直播水稻萌发、生长及产量的影响[D]. 武汉: 华中农业大学, 2015.
[75]	肖武. 水稻抗寒种衣剂的生物学效应及应用效果评价[D]. 长沙: 湖南农业大学, 2006.
[76]	梅俊豪. 种子丸粒化在水稻湿直播上的应用初探[D]. 武汉: 华中农业大学, 2017.
[77]	HATTON W, BAKER A M. Calcium peroxide as a seed coating material for padi rice[J]. Plant and Soil, 1987, 99: 379-386.