A field experiment was conducted at Luzhou City in 2016—2017, in order to explore the effects of plant density and nitrogen management on yield formation and quality of hybrid mid-season rice in rice-ratoon rice system. The results showed that, under the same transplanting density, the yield of main rice and ratoon rice increased first and then decreased with the increase of nitrogen application rate. Under the condition of high density (D3, 2.4×105 hills/hm2), the nitrogen application amount under N2 treatment (precision fertilizer based on the leaf measured, PFLM)was decreased by 7.8% on average compared with N1 treatment(N application rate was 225 kg/hm2), but the yield in the first season, the yield in regeneration season and the total yield in the two seasons were increased by 4.9%, 15.4% and 6.8% respectively compared with N1 treatment. With the increase of transplanting density, the number of effective panicles in the first season and regeneration season of hybrid rice showed an increasing trend, the grain number per spike showed a decreasing trend (except ratoon rice in 2017). In the first season, the effective panicle number of N1 treatment was 5.7% higher than that of N2 treatment on average, but the grain number per panicle and spikelets were 9.2% and 3.1% lower than that of N2 treatment, respectively; in the regeneration season, the effective panicle number, grain number per panicle and spikelets of N2 treatment were 10.2%, 9.0% and 18.2% higher than that of N1 treatment, respectively. Compared with the N1 treatment, the protein content of N2 treatment was increased by 6.9% on average in the first season, but decreased by 1.8% in the regeneration season. The processing quality, appearance quality and grain size in the first season and regenerated season were less different between different treatments. In general, the brown rice rate, milled rice rate, head rice rate with D3N2 combination were relatively high, while the chalky grain rate and chalkiness degree were relatively low. Therefore, it is suggested that in the ratoon rice production system in the study area, the transplanting density of 2.4×105 hills/hm2 with PFLM technique were more suitable.