The purpose of this study was to preliminary identify and evaluate the cold tolerance of different fragrant rice varieties at seedling stage, and to screen out the fragrant rice varieties with strong cold tolerance at seeding stage, finally to provide the basis for the hypothermia injury mechanism of fragrant rice at seedling stage. Taking super inbred fragrant rice variety Yuxiangyouzhan as control, the effects of low temperature on seedling quality, photosynthetic physiological characteristics, chlorophyll fluorescence, antioxidant enzyme activity and malondialdehyde(MDA) contents of seven inbred fragrant rice varieties (Basmati, Guixiangzhan, Nongxiang 18, Xiangwanxian 13, Xiangyaxiangzhan, Zhongxiang 1, Meixiangzhan 2) were studied. The survival rate of fragrant rice varieties Guixiangzhan, Nongxiang 18, Xiangwanxian 13, Zhongxiang 1 and Meixiangzhan 2 had no significant difference with the control, while the survival rate of Basmati and Xiangyaxiangzhan was significantly reduced. The variation range of dry weight of fragrant rice varieties was 14.84%~39.84%, and that of fresh weight was 2.17%~28.34%. With the low temperature treatment, Nongxiang 18, Xiangwanxian 13 and Zhongxiang 1 had higher survival rate than other cultivars, due to their dry weight and fresh weight were less affected. Moreover, the cultivars with higher survival rate showed some advantages in photosynthetic physiological characteristics, fluorescence characteristics and antioxidant enzyme activities. Guixiangzhan, Nongxiang 18, Xiangwanxian 13, Zhongxiang 1 and Meixiangzhan 2 showed strong low temperature resistance, while Basmati and Xiangyaxiangzhan were poor.