The development of environmentally friendly disease prevention and control technology is conducive to the healthy and sustainable development of rice industry. To clarify the control efficacy of combined application of jinggangmycin and nano-silicon fertilizer against rice sheath blight, as well as its effects on photosynthetic characteristics and defense enzyme activities in rice, an experiment was conducted using the rice cultivar Yexiangyou 669 as the test material, aiming to screen for the optimal combined application scheme. The results showed that, compared to the control (CK, sprayed with an equal volume of sterile water), the combined application of jinggangmycin and nano-silicon fertilizer significantly enhanced the photosynthetic characteristics of rice. Among all treatments, the T3 treatment (spraying 5% validamycin A wettable powder 125 g+nano-silicon fertilizer 700 mL) demonstrated the best performance. Under this treatment condition, the total chlorophyII content, the net photosynthetic rate, stomatal conductance, intercellular CO2 concentration and transpiration rate of rice were 4.69 mg/g, 14.9 μmol/(m2·s), 0.47μmol/(m2·s), 268.7 μmol/mol and 5.3 mmol/(m2·s) respectively. Chlorophyll fluorescence parameters, including potential photochemical efficiency (Fv/Fo), maximum photochemical efficiency of PSII (Fv/Fm), photochemical quenching coefficient (qP), and non-photochemical quenching coefficient (NPQ), were significantly higher than those in other treatments. The activities of antioxidant defense enzymes, such as superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL), reached their highest values, with specific activities of 513.3, 875.1, 117.7, and 47.9 U/(g·h), respectively. Meanwhile, under this treatment condition, the disease index and control effect of rice were 2.18 and 76.2 %, respectively, and the prevention and control of rice sheath blight reached the best effect. In conclusion, the T3 treatment improved chlorophyll content, photosynthetic characteristics, and chlorophyll fluorescence parameters in rice, activated antioxidant defense enzymes, and demonstrated excellent control efficacy against rice sheath blight.