中国稻米

中国稻米 ›› 2026, Vol. 32 ›› Issue (4): 102-107.DOI: 10.3969/j.issn.1006-8082.2026.04.017

• 品种与技术 • 上一篇    下一篇

籼稻恢复系R382苗期耐冷生理机制与基因表达特性分析

张静1(), 朱童童1, 徐佳琛1, 李连州1, 王昱1, 张桂莲1, 邓化冰1, 卢学丹1,*(), 唐文帮1,2,*()   

  1. 1 湖南农业大学 农学院, 长沙 410128
    2 湖南杂交水稻研究中心, 长沙 410125
  • 收稿日期:2026-03-12 出版日期:2026-07-20 发布日期:2026-07-14
  • 通讯作者: *luxuedan@hunau.edu.com;
    tangwenbang@163.com
  • 作者简介:

    第一作者：13364950541@163.com

  • 基金资助:
    岳麓山实验室种业专项-重大新品种培育项目(YLS-2025-ZY02001);岳麓山实验室种业专项-青年项目(YLS-2025-ZY04023);湖南省科技创新计划项目(2024RC3183);长沙市自然科学基金(kq2502269)

Analysis of Chilling Tolerance Physiological Mechanism and Gene Expression Characteristics of Indica Rice Restorer Line R382 at the Seedling Stage

ZHANG Jing1(), ZHU Tongtong1, XU Jiachen1, LI Lianzhou1, WANG Yu1, ZHANG Guilian1, DENG Huabing1, LU Xuedan1,*(), TANG Wenbang1,2,*()   

  1. 1 College of Agronomy, Hunan Agricultural University, Changsha 410128, China
    2 Hunan Hybrid Rice Research Center, Changsha 410125, China

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摘要：

本研究对26个杂交籼稻恢复系进行苗期耐冷性评价,鉴定出强耐冷性恢复系R382;以大面积推广应用但耐冷性较弱的恢复系9311为对照,通过比较R382与9311耐冷相关生理指标及低温应答基因表达水平的差异,揭示了R382苗期耐冷的生理机制。在相同生长环境下,系统分析了常温及低温胁迫条件下,R382和9311水稻幼苗叶片的多项生理指标变化,包括活性氧(过氧化氢、超氧阴离子)水平、丙二醛含量、抗氧化酶活性、相对电导率及渗透保护物质脯氨酸含量等。同时,采用实时定量PCR技术,检测了苗期低温胁迫下抗氧化酶编码基因(过氧化氢酶OsCATB、超氧化物歧化酶OsFe-SOD、抗坏血酸过氧化物酶OsAPX1)及富含脯氨酸蛋白编码基因OsPRP1的表达水平。结果表明,低温胁迫后,R382的抗氧化酶编码基因表达水平显著高于9311,抗氧化酶活性更强,活性氧及毒性化合物丙二醛积累量更少;同时,其细胞内渗透保护物质脯氨酸含量显著升高,细胞相对电导率较低,这一系列生理及分子层面的差异共同赋予了R382更强的苗期耐冷性。

关键词: 籼稻, 恢复系, R382, 低温胁迫, 生理特性, 基因表达

Abstract:

In this study, 26 indica hybrid rice restorer lines were evaluated for cold tolerance at the seedling stage, and the strongly cold-tolerant restorer line R382 was identified. Using the widely cultivated but relatively cold-sensitive restorer line 9311 as a control, the physiological mechanisms underlying the cold tolerance of R382 at the seedling stage were revealed by comparing differences in cold-related physiological indices and low-temperature-responsive gene expression levels between R382 and 9311. Under identical growth conditions, changes in several physiological parameters of rice seedlings (leaves) of R382 and 9311 were systematically analyzed under both normal temperature and low-temperature stress, including levels of reactive oxygen species (hydrogen peroxide, superoxide anion), malondialdehyde (MDA) content, antioxidant enzyme activities, relative electrolyte leakage, and the osmoprotectant proline content. In addition, real-time quantitative PCR (qRT-PCR) was used to measure the expression levels of genes encoding antioxidant enzymes catalase (OsCATB), iron-superoxide dismutase (OsFe-SOD), ascorbate peroxidase (OsAPX1) and the proline-rich protein gene OsPRP1 under low-temperature stress at the seedling stage. The results showed that after low-temperature treatment, the expression levels of antioxidant enzyme-coding genes in R382 were significantly higher than those in 9311, accompanied by stronger antioxidant enzyme activities, lower accumulation of reactive oxygen species and the toxic compound MDA, and a marked increase in intracellular proline content, along with lower relative electrolyte leakage. These combined physiological and molecular differences contribute to the stronger cold tolerance of R382 at the seedling stage.

Key words: indica rice, restorer line, R382, low-temperature stress, physiological characteristics, gene expression

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