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利用分子标记鉴定长粒粳稻品种粒形相关基因的基因型

  1. 1 湖北省农业科学院粮食作物研究所/粮食作物种质创新与遗传改良湖北省重点实验室,武汉 430064;2 武汉大学研究生院,武汉 430072;3 长江大学/主要粮食作物产业化湖北省协同创新中心,湖北 荆州 434025
  • 出版日期:2020-11-20 发布日期:2020-11-20
  • 通讯作者: 游艾青
  • 基金资助:
    “十三五”国家重点研发计划项目(2018YFD0301305;2017YFD0100406)

Genotyping of 10 Grain Shape Genes in Seven Long-grain Japonica Rice Varieties by Molecular Marker

  • Online:2020-11-20 Published:2020-11-20

摘要: 选取7个具有代表性的长粒粳稻品种为供试材料,以短圆粒粳稻品种日本晴为对照,利用分子标记检测方法,对GS3、LGY3、qGL3、GL7、SLG7、TGW6、GS9等7个粒长基因和GW8、GW5、GS5等3个粒宽基因进行基因型分析。结果发现,7个长粒粳品种都具有gs3长粒等位基因,鄂香2号、CLG-YZ、CLG-HZ、CLG-HB、CLG-JX具有GL7和SLG7长粒等位基因,稻花香2号具有lgy3长粒等位基因,CLG-HZ具有GW5宽粒等位基因,鄂香2号具有GW8宽粒等位基因。本研究结果不仅对现有长粒粳品种粒型基因型有一个较全面的认识,还为今后利用分子标记辅助选育长粒粳新品种和亲本选择提供了重要的基因型信息。

关键词: 水稻, 长粒粳稻, 粒型基因, 分子标记, 基因型

Abstract: Seven representative long-grain japonica rice varieties were selected as test materials, and short-grain japonica rice varieties Nipponbare was used as control. Seven grain length-related genes including GS3, LGY3, qGL3, GL7, SLG7, TGW6 and GS9, and three grain width-related genes including GW8, GW5 and GS5 were tested by molecular marker. The results showed that, seven long-grain japonica varieties all have the gs3 long-grain allele, Exiang 2, CLG-YZ, CLG-HZ, CLG-HB, CLG-JX have the GL7 and SLG7 long-grain alleles, Daohuaxiang 2 has the lgy3 long-grain allele, CLG-HZ has the GW5 wide-grain gene, and Exiang 2 has the GW8 wide-grain allele. This study not only has a more comprehensive understanding of the genotype of existing long-grain japonica varieties, but also provides important genotype information in selecting and cultivating new long-grain japonica varieties via molecular marker-assisted selection in future.

Key words: rice, long grain japonica rice, grain shape gene, molecular marker, genotype

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