中国稻米

中国稻米 ›› 2023, Vol. 29 ›› Issue (3): 56-61.DOI: 10.3969/j.issn.1006-8082.2023.03.010

• 专论与研究 • 上一篇    下一篇

丛枝菌根真菌缓解水稻盐碱胁迫的生理特性研究

张思华1,2,#, 弥春霞1,#, 虞轶俊3, 刘国群4, 朱春权2, 田文昊2, 朱练峰2, 曹小闯2, 张均华2,*(), 孔亚丽2,*()   

  1. 1牡丹江师范学院，黑龙江 牡丹江 157011
    2中国水稻研究所/水稻生物育种全国重点实验室，杭州 311400
    3浙江省耕地质量与肥料管理总站，杭州 310020
    4衢州市柯城区美丽乡村建设中心，浙江 衢州 324002
  • 收稿日期:2022-10-27 出版日期:2023-05-20 发布日期:2023-05-23
  • 通讯作者: 张均华,孔亚丽
  • 作者简介:第一联系人：

    #共同第一作者

  • 基金资助:
    国家自然科学基金(31901452);国家自然科学基金(31872857);国家自然科学基金(31771733);浙江省“三农九方”项目(2022SNJF001);水稻生物育种全国重点实验室自主课题(2020ZZKT10402);中央级公益性科研院所基本科研业务专项(CPSIBRF-CNRRI-202120)

Physiological Characteristics of Arbuscular Mycorrhizal Fungi in Alleviating Saline Alkali Stress in Rice

ZHANG Sihua1,2,#, MI Chunxia1,#, YU Yijun3, LIU Guoqun4, ZHU Chunquan2, TIAN Wenhao2, ZHU Lianfeng2, CAO Xiaochuang2, ZHANG Junhua2,*(), KONG Yali2,*()   

  1. 1Mudanjiang Normal University, Mudanjiang, Heilongjiang 157011, China
    2China National Rice Research Institute/State Key Laboratory of Rice Biology, Hangzhou 311400, China
    3Zhejiang Cultivated Land Quality and Fertilizer Administration Station, Hangzhou 310020, China
    4Beautiful Countryside Construction Center of Kecheng District, Quzhou, Zhejiang 324002, China
  • Received:2022-10-27 Online:2023-05-20 Published:2023-05-23
  • Contact: ZHANG Junhua, KONG Yali
  • About author:First author contact:

    #Co-first author

RichHTML

PDF

摘要：

为探明丛枝菌根真菌（arbuscular mycorrhizal fungi，AMF）对盐碱胁迫下不同水稻品种生长和生理特性的影响，采用温室盆栽试验，在盐碱和非盐碱条件下设接种根内根孢囊霉（Rhizophagus intraradices）和不接种处理，研究了耐盐碱水稻品种津源85和盐碱敏感水稻品种辽粳763的根系菌根侵染率、生长特性、抗氧化系统及渗透调节物质的响应情况。结果表明，盐碱胁迫会显著抑制水稻的生长，接种AMF后可提高水稻株高和干物质量，且以津源85效果更好；盐碱胁迫会导致水稻植株MDA和H2O2含量增加，接种AMF后会显著提高SOD、POD、CAT和APX的活性，同时 H2O2和MDA含量显著降低；此外，AMF还可以通过提高盐碱胁迫条件下植株的脯氨酸、可溶性糖和可溶性蛋白含量来缓解渗透胁迫。可见，AMF可在一定程度上缓解因盐碱胁迫造成的生理生长活性的抑制，通过调控抗氧化酶系统和渗透调节物质来提高水稻对盐碱胁迫适应性，且对不同品种所发挥的效果不完全相同。

关键词: 水稻, 盐碱胁迫, 丛枝菌根真菌, 抗氧化系统, 渗透物质

Abstract:

To explore the effects of arbuscular mycorrhizal fungi (AMF) inoculation on the growth of different rice varieties under saline alkali stress, the pot culture was conducted in greenhouse to study the root mycorrhizal infection rate, growth characteristics, antioxidant system and osmoregulation responses between saline alkali tolerant rice variety Jinyuan 85 and sensitive rice variety Liaojing 763 under saline alkali and non saline alkali conditions with or without inoculating Rhizophagus intraradices. The results showed that salt alkali stress could significantly inhibit the growth of rice, and AMF inoculation could increase the plant height and dry weight, especially in Jinyuan 85. Moreover, the content of MDA and H2O2 in rice plants increased under saline alkali stress. After inoculation with AMF, the activities of SOD, POD, CAT and APX were significantly increased, while the content of H2O2 and MDA were significantly decreased. In addition, AMF could also alleviate osmotic stress by increasing proline, soluble sugar and soluble protein content of plants under stress. It can be seen that AMF can alleviate the inhibition of physiological growth activity caused by saline alkali stress to a certain extent, and improve the adaptability of rice to saline alkali stress by regulating antioxidant enzyme system and osmoregulation substances, and the effect of AMF on different varieties is not the same.

Key words: rice, saline alkali stress, arbuscular mycorrhizal fungi, antioxidant system, osmotic substances

中图分类号: