Distribution and Resistance Analysis of Blast Resistance Genes in Rice Varieties from the Huang-Huai-Hai Region

doi:10.3969/j.issn.1006-8082.2026.03.012

China Rice ›› 2026, Vol. 32 ›› Issue (3): 71-75.DOI: 10.3969/j.issn.1006-8082.2026.03.012

• Special Thesis & Basic Research • Previous Articles Next Articles

Distribution and Resistance Analysis of Blast Resistance Genes in Rice Varieties from the Huang-Huai-Hai Region

ZHANG Qiaoling¹^,²(), GAO Farui¹, GAO Guoliang¹, LOU Like², HUANG Jingfen²^,³, HUANG Xincheng¹, QIAO Weihua²^,³^,^*()

¹ Jining Academy of Agricultural Sciences, Jining, Shandong 272075, China
² National Nanfan Research Institute（Sanya）, Chinese Academy of Agricultural Sciences, Sanya, Hainan 572024, China
³ Institute of Crop, SciencesChinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2025-10-22 Online:2026-05-20 Published:2026-05-11
Contact: QIAO Weihua
About author:First author contact:
1st author: zhangql2021@163.com

黄淮海稻区水稻品种抗稻瘟病基因的分布与抗性分析

张巧玲¹^,²(), 高发瑞¹, 高国良¹, 娄理科², 黄婧芬²^,³, 黄信诚¹, 乔卫华²^,³^,^*()

¹ 山东省济宁市农业科学研究院，山东济宁 272075
² 三亚中国农业科学院国家南繁研究院，海南三亚 572024
³ 中国农业科学院作物科学研究所，北京 100081

通讯作者: 乔卫华
作者简介:第一联系人：
第一作者：zhangql2021@163.com
基金资助:
三亚市科技“繁星”专项科研项目(2024KJFX016);山东省水稻产业技术体系“济宁综合试验站”建设项目(SDAIT-17-17);山东省水稻产业技术体系“济宁综合试验站”建设项目(SDAIT-17-11)

Abstract

Abstract:

In this study, functional molecular markers of eight blast resistance genes—Pigm, Pi2, Pid3-A4, Ptr, Pita, Pi63, Pi9, and bsr-d1—were used to detect resistance genes in 90 japonica rice cultivars popularized in the Huang-Huai-Hai rice region. Resistance identification was also conducted via artificial inoculation with a mixture of Magnaporthe oryzaeraces under field conditions. The results showed that only four resistance genes—Pita, Pi2, Pigm, and Pid3-A4—were detected in the tested cultivars. Materials carrying the Pigm gene alone generally exhibited broad-spectrum resistance, while the combination of Pita and Pi2 genes conferred broad-spectrum and durable resistance, although single-gene-mediated resistance was unstable. Cultivars from Shandong and Jiangsu provinces showed better overall blast resistance, with Jiangsu cultivars displaying higher polymorphism of the detected resistance genes and more diverse gene combinations. This study clarifies the distribution of blast resistance genes in main japonica rice cultivars from the Huang-Huai-Hai region and its correlation with disease resistance, providing a theoretical basis and germplasm resources for pyramiding superior genes in resistance breeding.

Key words: rice, Huang-Huai-Hai rice region, blast resistance gene, molecular marker

摘要：

本研究利用Pigm、Pi2、Pid3-A4、Ptr、Pita、Pi63、Pi9和bsr-d1等8个抗稻瘟病基因的功能性分子标记，对黄淮海稻区推广的90个粳稻育成品种进行抗性基因检测，并结合田间人工接种稻瘟病混合小种进行抗性鉴定。结果表明，在供试品种中仅检出Pita、Pi2、Pigm和Pid3-A4等4个抗病基因。携带Pigm单基因的材料普遍表现广谱抗性；Pita与Pi2组合则呈现广谱且持久的抗病效果，而单基因抗性则不稳定。山东和江苏品种的抗病性整体较优，其中江苏品种所含抗病基因多态性更高，基因组合类型也更丰富。本研究明确了黄淮海稻区主栽粳稻品种中稻瘟病抗性基因的分布及其与抗性的关联，为优异基因聚合与抗病育种提供了理论依据和种质资源。

关键词: 水稻, 黄淮海稻区, 抗稻瘟病基因, 分子标记

CLC Number:

ZHANG Qiaoling, GAO Farui, GAO Guoliang, LOU Like, HUANG Jingfen, HUANG Xincheng, QIAO Weihua. Distribution and Resistance Analysis of Blast Resistance Genes in Rice Varieties from the Huang-Huai-Hai Region[J]. China Rice, 2026, 32(3): 71-75.

张巧玲, 高发瑞, 高国良, 娄理科, 黄婧芬, 黄信诚, 乔卫华. 黄淮海稻区水稻品种抗稻瘟病基因的分布与抗性分析[J]. 中国稻米, 2026, 32(3): 71-75.

Figures/Tables 4

References 23

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Distribution and Resistance Analysis of Blast Resistance Genes in Rice Varieties from the Huang-Huai-Hai Region

黄淮海稻区水稻品种抗稻瘟病基因的分布与抗性分析

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