Effects of Florigen RFT1 for Promoting Flowering in Rice

doi:10.3969/j.issn.1006-8082.2020.03.005

Abstract

Abstract: Shortening the growth period of early-season rice varieties is one of the important breeding objectives in the double-season rice region in southern China. In this study, near isogenic lines （NILs） and NIL-F2 populations that segregated the florigen gene RFT1 in the early-heading genetic background were developed using early-season rice variety Zhenshan 97 and middle-season rice variety Miyang 46 as the parents. The populations were tested for heading date and yield traits under natural long-day conditions. The results showed that the Miyang 46 allele of RFT1 could promote heading date and had a slight negative effect on yield traits. The additive effects on heading date ranged from 0.84 to 1.21 days for heading date, explaining 10.46% to 18.62% of the phenotypic variances. The additive effects estimated in two-year's trials using the NIL population were 3.06 and 2.02 for the number of spikelets per panicle, 2.98 and 1.48 for the number of grains per panicle, 0.12 g and 0.08 g for 1 000-grain weight, and 0.63 g and 0.38 g for grain yield per plant, with the phenotypic variances explained ranging from 0.99% to 2.76%. Our results indicated that the Miyang 46 allele of RFT1 could further shorten the growth period of early-season varieties with slight penalty in grain yield, providing more abundant growth time for late-season rice which could increase the production potential of double-season rice.

Key words: rice, heading date, florigen, minor effect, early maturity, pleiotropism

摘要： 进一步缩短早稻的生育期是我国南方双季稻区的重要育种目标之一。本研究以早籼稻珍汕97和中籼稻密阳46为亲本，构建了珍汕97早熟型遗传背景的近等基因系（NIL）和NIL-F2群体，分析成花素基因RFT1在早稻遗传背景和自然长日照种植条件下的遗传作用。结果表明，与RFT1的珍汕97等位基因相比，密阳46等位基因能够显著促进抽穗，同时对产量性状呈轻微负向作用。在2个群体中，RFT1对抽穗期的加性效应为0.84~1.21 d，贡献率为10.46%~18.62%。在NIL群体2年重复试验中，每穗总粒数、每穗实粒数、千粒重和单株产量的加性效应分别为3.06粒和2.02粒、2.98粒和1.48粒、0.12 g和0.08 g、0.63 g和0.38 g，贡献率在0.99%~2.76%之间。这些结果显示，密阳46的RFT1等位基因能够在较低产量代价下缩短早稻的生育期，为晚稻提供更充裕的生长时间，进而提高双季稻的生产潜能。

关键词: 水稻, 抽穗期, 成花素基因, 微效作用, 早熟, 多效性

CLC Number:

S511

王世林,张振华,朱玉君,樊叶杨,庄杰云*. 水稻成花素基因RFT1对抽穗期的促进作用[J]. 中国稻米, DOI: 10.3969/j.issn.1006-8082.2020.03.005 .

References

[1] 段居琦，周广胜. 中国双季稻种植区的气候适宜性研究[J]. 中国农业科学，2012，45（2）：218-227. [2] 邹应斌，戴魁根. 湖南发展双季稻生产的优势[J]. 作物研究，2008，22（4）：209-213. [3] 徐春春，纪龙，陈中督，等. 2017年我国水稻产业形势分析及2018年展望[J]. 中国稻米，2018，24（2）：1-3. [4] 徐春春，纪龙，陈中督，等. 2018年我国水稻产业形势分析及2019年展望[J]. 中国稻米，2019，25（2）：1-3. [5] 肖国樱，肖友伦，李锦江，等. 高效是当前水稻育种的主导目标[J]. 中国水稻科学，2019，33（4）：287-292. [6] HORI K, MATSUBARA K, YANO M. Genetic control of flowering time in rice: integration of mendelian genetics and genomics[J]. Theor Appl Genet, 2016, 129(12）: 2 241-2 252. [7] KOMIYA R, IKEGAMI A, TAMAKI S, et al. Hd3a and RFT1 are essential for flowering in rice[J]. Development, 2008, 135（4）: 767-774. [8] KOMIYA R, YOKOI S, SHIMAMOTO K. A gene network for long-day flowering activates RFT1 encoding a mobile flowering signal in rice[J]. Development, 2009, 136（20）: 3 443-3 450. [9] YANO M, KATAYOSE Y, ASHIKARI M, et al. Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS[J]. Plant Cell, 2000, 12（12）: 2 473-2 483. [10] DOI K, IZAWA T, FUSE T, et al. Ehd1, a B-type response regulator in rice, confers short-day promotion of flowering and controls FT-like gene expression independently of Hd1[J]. Genes Dev, 2004, 18（8）: 926-936. [11] GAO H, JIN M, ZHENG X M, et al. Days to heading 7, a major quantitative locus determining photoperiod sensitivity and regional adaptation in rice[J]. Proc Natl Acad Sci USA, 2014, 111（46）: 16 337-16 342. [12] YAN W, LIU H, ZHOU X, et al. Natural variation in Ghd7.1 plays an important role in grain yield and adaptation in rice[J]. Cell Res, 2013, 23（7）: 969-971. [13] XUE W, XING Y, WENG X, et al. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice[J]. Nat Genet, 2008, 40（6）: 761-767. [14] WEI X, XU J, GUO H, et al. DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously[J]. Plant Physiol, 2010, 153（4）: 1 747-1 758. [15] 徐俊锋，魏祥进，江玲，等. 我国部分早籼品种及杂交早籼骨干亲本抽穗期遗传分析[J]. 中国水稻科学，2010，24（3）：215-222. [16] ZHU Y J, FAN Y Y, WANG K, et al. Rice Flowering Locus T 1 plays an important role in heading date influencing yield traits in rice[J]. Sci Rep, 2017, 7（1）: 4 918. [17] 陈俊宇，王凯，龚俊义，等. RFT1与Hd1所在区间对水稻抽穗期、株高和千粒重的作用[J]. 中国水稻科学，2013，27（2）：117-121. [18] ZHANG H W, FAN Y Y, ZHU Y J, et al. Dissection of the qTGW1.1 region into two tightly-linked minor QTLs having stable effects for grain weight in rice[J]. BMC Genet, 2016, 17（1）: 98. [19] ZHENG K, HUANG N, BENNETT J, et al. PCR-based marker-assisted selection in rice breeding[M]. IRRI Discussion Paper Series No.12. Manila: International Rice Research Institute, 1995. [20] CHEN X, TEMNYKH S, XU Y, et al. Development of a microsatellite framework map providing genome-wide coverage in rice[J]. Theor Appl Genet, 1997, 95（4）: 553-567. [21] WANG S, BASTEN C J, ZENG Z B. Windows QTL Cartographer 2.5[M]. Raleigh, NC, USA: Department of Statistics, North Carolina State University, 2012. [22] SAS Institute Inc. SAS/STAT User’s Guide[M]. Cary, NC: SAS Institute, 1999. [23] DAI W M, ZHANG K Q, WU J R, et al. Validating a segment on the short arm of chromosome 6 responsible for genetic variation in the hull silicon content and yield traits of rice[J]. Euphytica, 2008, 160（3）: 317-324. [24] NEMOTO Y, NONOUE Y, YANO M, et al. Hd1, a CONSTANS ortholog in rice, functions as an Ehd1 repressor through interaction with monocot-specific CCT-domain protein Ghd7[J]. Plant J, 2016, 86（3）: 221-233. [25] ZHANG Z H, ZHU Y J, WANG S L, et al. Importance of the interaction between heading date genes Hd1 and Ghd7 for controlling yield traits in rice[J]. Int J Mol Sci, 2019, 20（3）: 516. [26] CHEN J Y, GUO L, MA H, et al. Fine mapping of qHd1, a minor heading date QTL with pleiotropism for yield traits in rice[J]. Theor Appl Genet, 2014, 127（11）: 2 515-2 524. [27] WU W, ZHENG X M, LU G, et al. Association of functional nucleotide polymorphisms at DTH2 with the northward expansion of rice cultivation in Asia[J]. Proc Natl Acad Sci USA, 2013, 110（8）: 2 775-2 780. [28] BIAN X F, LIU X, ZHAO Z G, et al. Heading date gene, dth3 controlled late flowering in O. Glaberrima Steud. by down-regulating Ehd1[J]. Plant Cell Rep, 2011, 30（12）: 2 243-2 254. [29] MATSUBARA K, OGISO-TANAKA E, HORI K, et al. Natural variation in Hd17, a homolog of Arabidopsis ELF3 that is involved in rice photoperiodic flowering[J]. Plant Cell Physiol, 2012, 53（4）: 709-716. [30] SHIBAYA T, HORI K, OGISO-TANAKA E E, et al. Hd18, encoding histone acetylase related to Arabidopsis FLOWERING LOCUS D, is involved in the control of flowering time in rice[J]. Plant Cell Physiol, 2016, 57（9）: 1 828-1 838. [31] CHEN L, ZHENG Z, WU W, et al. Fine mapping of DTH3b, a minor heading date QTL potentially functioning upstream of Hd3a and RFT1 under long-day conditions in rice[J]. Mol Breed, 2015, 35（11）: 206. [32] ZHONG Z, WU W, WANG H, et al. Fine mapping of a minor-effect QTL, DTH12, controlling heading date in rice by up-regulation of florigen genes under long-day conditions[J]. Mol Breed, 2014, 34（2）: 311-322. [33] TAKEUCHI Y, EBITANI T, YAMAMOTO T, et al. Development of isogenic lines of rice cultivar koshihikari with early and late heading date by marker-assisted selection[J]. Breed Sci, 2006, 56（4）: 405-413. [34] ZHAO J, CHEN H, REN D, et al. Genetic interactions between diverged alleles of Early heading date 1 （Ehd1） and Heading date 3a （Hd3a）/ RICE FLOWERING LOCUS T1 （RFT1） control differential heading and contribute to regional adaptation in rice[J]. New Phytol, 2015, 208（3）: 936-948. [35] FUJINO K, OBARA M, IKEGAYA T. Establishment of adaptability to the northern-limit of rice production[J]. Mol Genet Genomics, 2019, 294（3）: 729-737.