Rice germplasm resource is one of the most important bases for breeding. Cold resistance of rice cultivar in Heilongjiang province is a critical characteristic for adapting to cold climate. In this study, the authors evaluated 888 rice germplasm resources for cold tolerance at seedling stage under natural low temperature happened in 2017 of Heilongjiang province, which could provide a good material reference for cold resistant breeding. The results showed that 804 accessions were classed into the high-level tolerance (Grade 1) at seedling stage, accounting for 90.5% of total accessions. Among them, 128 accessions seedling survival rate was 100%, in which Heilongjiang, Jilin, Liaoning, Ningxia, Jiangsu, Japan, Korea, India has 56, 40, 8, 4, 1, 16, 4, 1 accession, respectively, accounting for 14.6% of total accessions; 40 accessions were classed into Grade 2, accounting for 4.5% of total accessions; 23 accessions were classed into Grade 3, accounting for 2.6% of total accessions; 11 accessions were classed into Grade 4, accounting for 1.2% of total accessions; 5, 3, 2 accessions were classed into Grade 5, Grade 6, Grade 7, accounting for 0.6%, 0.3%, 0.2%, respectively.