中国稻米

中国稻米 ›› 2021, Vol. 27 ›› Issue (6): 47-49.DOI: 10.3969/j.issn.1006-8082.2021.06.009

• 专论与研究 • 上一篇    下一篇

浸种剂对水稻种子微生物群落结构的影响

刘元辉(), 徐春梅, 陈松, 褚光, 章秀福, 王丹英*()   

  1. 中国水稻研究所,杭州 310006
  • 收稿日期:2021-05-09 出版日期:2021-11-20 发布日期:2021-11-20
  • 通讯作者: 王丹英
  • 作者简介:

    第一作者：liuyuanhui@caas.cn

  • 基金资助:
    中央级公益性科研院所基本科研业务费专项（CPSIBRF-CNRRI-202119）

Effects of Seed Soaking Agent on the Structure of Rice Seed Microbial Community

Yuanhui LIU(), Chunmei XU, Song CHEN, Guang CHU, Xiufu ZHANG, Danying WANG*()   

  1. China National Rice Research Institute, Hangzhou 310006, China
  • Received:2021-05-09 Online:2021-11-20 Published:2021-11-20
  • Contact: Danying WANG
  • About author:

    1st author: liuyuanhui@caas.cn

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摘要：

以常规粳稻秀水134为材料,设置清水浸种（CK）和咪酰胺浸种（Soak）两个处理,采用16S rRNA扩增子测序和分离培养等方法,研究浸种剂咪酰胺对水稻种子微生物群落结构的影响。结果表明,与CK相比,Soak处理水稻种子表面真菌数目显著减少,而对水稻种子内细菌群落多样性和组成,包括细菌群落Shannon 指数、Chao1 指数、Observed OTUs、β多样性以及优势菌群相对丰度没有显著影响。可见,在农业生产中,咪酰胺浸种作为防治水稻种传病害的措施对种子内生菌群没有显著影响。

关键词: 水稻, 浸种, 种子微生物, 群落结构

Abstract:

The effects of seed soaking agent prochloraz on the structure of microbial community in rice seeds were studied by setting seed soaking with water （CK） and seed soaking with prochloraz （Soak）, 16S rRNA amplicon sequencing and isolation culture methods in conventional japonica rice Xiushui 134. The results showed that compared with CK, the number of fungi on rice seed surface of Soak was significantly reduced. However, Soak had no significant effect on the diversity and composition of bacterial communities in rice seeds, including Shannon index, Chao1 index, Observed OTUs, β diversity and relative abundance of dominant bacteria. Thus, in agricultural production, prochloraz soaking as a measure to control rice seed-borne diseases has no significant effect on seed endophytic bacteria.

Key words: rice, seed soak, seed microbiome, community structure

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