籼稻品种滇屯502苗期耐低温的生理与分子特性分析

doi:10.3969/j.issn.1006-8082.2025.03.0012

中国稻米 ›› 2025, Vol. 31 ›› Issue (3): 75-80.DOI: 10.3969/j.issn.1006-8082.2025.03.0012

籼稻品种滇屯502苗期耐低温的生理与分子特性分析

施怡彤¹^,^#(), 雍雨¹^,^#, 李秋平², 马雯清², 徐笑宇¹, 袁兴巧¹, 毕苧¹, 白晓清¹, 雷文洪¹, 文建成²^,^*(), 李丹丹²^,^*()

¹云南农业大学农学与生物技术学院，昆明 650201
²云南农业大学稻作研究所，昆明 650201

收稿日期:2024-08-05 出版日期:2025-05-20 发布日期:2025-05-14
通讯作者: * jcwen1117@163.com
lidanzaizhe@163.com
作者简介:
^#共同第一作者：m13988499100@163.com
基金资助:
云南省重大科技专项(202402AE090026-04);云南省农业基础研究联合专项-面上项目(202301BD070001-121);云南省科技厅基础研究专项-面上项目(202201AT070263);建设面向南亚东南亚科技创新中心专项(202203AK140016);昆明市院士自由探索项目子课题(KJHZC-2022YS02);云南农业大学科研启动基金项目(KY2022-10)

Physiological and Molecular Characteristics of Low-Temperature-Tolerant Indica Rice Variety Diantun 502 during the Seedling Stage

SHI Yitong¹^,^#(), YONG Yu¹^,^#, LI Qiuping², MA Wenqing², XU Xiaoyu¹, YUAN Xingqiao¹, BI Ning¹, BAI Xiaoqing¹, LEI Wenhong¹, WEN Jiancheng²^,^*(), LI Dandan²^,^*()

¹College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China
²Rice Research Institute, Yunnan Agricultural University, Kunming 650201, China

Received:2024-08-05 Published:2025-05-20 Online:2025-05-14
Contact: * jcwen1117@163.com
lidanzaizhe@163.com
About author:
^#Co-first author: m13988499100@163.com

摘要/Abstract

摘要：

本研究以云南高海拔耐低温籼稻品种滇屯502及其近等位基因系但对低温敏感的滇屯506为研究对象，旨在探究低温胁迫条件下这2个水稻品种在生理代谢活动及低温响应基因表达方面的差异，从而为揭示滇屯502耐低温的分子机制提供理论依据。实验在相同环境下对两种水稻幼苗实施了低温处理，并系统分析了低温胁迫下水稻苗期叶片的多项生理指标变化，包括水分、叶绿素、脯氨酸（Pro）、丙二醛（MDA）、可溶性糖含量以及抗氧化酶系统的活性等。同时，本研究还检测了与水稻低温抗性相关的关键基因，如COLD1、OsRAN1、OsCAF1B、OsFAD8、OsAOX1a和OsPHY1的表达量变化。结果显示，滇屯502主要通过提高Pro含量、增强抗氧化酶活性以及上调OsCAF1B、OsFAD8、OsAOX1a和OsPHY1等基因表达水平来应对低温胁迫，从而保护苗期水稻免受低温伤害。

关键词: 籼稻, 滇屯502, 低温, 生理特性, 基因表达

Abstract:

This study selected the low-temperature-tolerant indica rice variety Diantun 502 and its near- allele low-temperatures sensitive line Diantun 506 as the research subjects, aiming to explore the differences in physiological metabolic activities and low-temperature response gene expression between these two rice varieties under low-temperature stress, thereby providing a theoretical basis for revealing the molecular mechanism of Diantun 502's low-temperature tolerance. The experiment subjected the seedlings of both rice varieties to low-temperature treatment under the same environmental conditions and systematically analyzed the changes in various physiological indicators in the leaves of rice seedlings under low-temperature stress, including water content, chlorophyll content, proline content, malondialdehyde content, soluble sugar content, and the activity of the antioxidant enzyme system. Additionally, this study also detected the changes in the expression levels of key genes related to rice low-temperature resistance, such as COLD1, OsRAN1, OsCAF1B, OsFAD8, OsAOX1a and OsPHY1. The results showed that Diantun 502 mainly respond to low temperature stress by increasing the content of proline, enhancing the activity of antioxidant enzymes, and upregulating the expression levels of genes such as OsCAF1B, OsFAD8, OsAOX1a, and OsPHY1, thereby protecting the rice seedlings from low-temperature damage.

Key words: indica rice, Diantun 502, low temperature, physiological characteristics, gene expression

中图分类号:

S511.04

施怡彤, 雍雨, 李秋平, 马雯清, 徐笑宇, 袁兴巧, 毕苧, 白晓清, 雷文洪, 文建成, 李丹丹. 籼稻品种滇屯502苗期耐低温的生理与分子特性分析[J]. 中国稻米, 2025, 31(3): 75-80.

SHI Yitong, YONG Yu, LI Qiuping, MA Wenqing, XU Xiaoyu, YUAN Xingqiao, BI Ning, BAI Xiaoqing, LEI Wenhong, WEN Jiancheng, LI Dandan. Physiological and Molecular Characteristics of Low-Temperature-Tolerant Indica Rice Variety Diantun 502 during the Seedling Stage[J]. China Rice, 2025, 31(3): 75-80.

图/表 4

表1 qRT-PCR引物序列

图1 滇屯502和滇屯506苗期低温胁迫0~5 d的表型检测结果

图2 滇屯502和滇屯506低温胁迫前后叶片生理指标变化

图3 滇屯502和滇屯506低温胁迫后6个水稻耐低温基因的表达量变化

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籼稻品种滇屯502苗期耐低温的生理与分子特性分析

Physiological and Molecular Characteristics of Low-Temperature-Tolerant Indica Rice Variety Diantun 502 during the Seedling Stage

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