中国稻米

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纳米碳与氮肥配施对水稻土壤养分和微生物群落结构的影响

  1. 黑龙江省农业科学院土壤肥料与环境资源研究所,哈尔滨 150086
  • 出版日期:2019-01-20 发布日期:2019-01-20
  • 基金资助:

    NAM长效专用肥料试验研究与示范(YS15B23);黑龙江省农业科技创新工程2014年度院级科研项目计划(2014QN023)

Effects of Nanocarbon Mixing with Nitrogen Fertilizer on Soil Microbial Community Structure and Nutrient Content

  • Online:2019-01-20 Published:2019-01-20

摘要:

采用盆栽试验,设置了不施氮肥(CK)、常规施肥(N2)、减施氮肥+纳米碳粉(N1+C)和常规施肥+纳米碳粉(N2+C)4个处理,研究了纳米碳配施氮肥对东北黑土养分和微生物群落结构的影响。结果表明,添加了纳米碳的处理土壤pH值和含水量均显著高于未添加纳米碳的处理,较N2处理土壤pH值平均高2.89%、土壤含水量平均高16.99%;添加纳米碳的处理土壤有机碳、速效磷和速效钾含量均显著高于未添加纳米碳的处理,分别高8.49%、7.49%和23.41%,且养分含量均以N1+C处理最高;土壤总PLFA含量表现为N2+C>N1+C>N2>CK,革兰氏阳性菌/阴性菌和细菌摩尔百分比表现为N1+C>N2+C>N2>CK,真菌和放线菌摩尔百分比表现为CK>N2>N1+C>N2+C。对土壤微生物群落结构进行主成分分析,结果表明,添加纳米碳使土壤微生物群落结构发生了改变,其中N2+C处理的影响最显著。可见,在农业生产中,纳米碳与氮肥配施对提高土壤养分含量、改善土壤微生物群落结构具有积极作用。

关键词: 水稻, 纳米碳, 土壤养分, 土壤微生物

Abstract:

The effects of nanocarbon mixing with nitrogen fertilizer on microbial community structure and nutrient content of soil were studied, based on a pot experiment with four treatments: no nitrogen fertilizer(CK), conventional fertilizer(N2), decreased-nitrogen fertilizer plus nanocarbon(N1+C) and conventional fertilizer plus nanocarbon(N2+C). The results showed that compared with N2 treatment, the pH value and water content of soil of the treatments adding nanocarbon were significantly increased by 2.89% and 16.99% respectively. The SOC, Olsen P, available K were significantly increased by 8.49%, 7.49%, 23.41% respectively. The PLFA content was in the order of N2+C > N1+C > N2 > CK. The GNB to GPB ratio and the percentage of bacterial moles were in the order of N1+C > N2+C > N2 > CK. The percentage of fungi and the percentage of actinomyces were in the order of CK > N2 > N1+C > N2+C. The principal analysis(PCA)results showed that adding nanocarbon obviously changed the microbial community structure, and N2+C was the most obviously changed treatment. In conclusion, nanocarbon mixing with nitrogen fertilizer could increase the nutrient and water content, improve the microbial biomass and structure of soil.

Key words: rice, nanocarbon, soil nutrient, soil microbe

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