Effects of Spermidine on Antioxidant Enzyme Activity and Na+ Homeostasis of Seedlings Roots of Huanghuazhan Rice under Salt Stress

doi:10.3969/j.issn.1006-8082.2023.02.009

China Rice ›› 2023, Vol. 29 ›› Issue (2): 43-47.DOI: 10.3969/j.issn.1006-8082.2023.02.009

• Special Thesis & Basic Research • Previous Articles Next Articles

Effects of Spermidine on Antioxidant Enzyme Activity and Na⁺ Homeostasis of Seedlings Roots of Huanghuazhan Rice under Salt Stress

HUANG Tingting¹^,², ZHENG Dianfeng¹^,², FENG Naijie¹^,², ZHAO Liming¹^,², ZHOU Hongkai¹^,², SHEN Xuefeng¹^,²

1. College of Coastal Agricultural Science, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China
2. South China Branch of National Saline Alkali Tolerant Rice Technology Innovation Center, Zhanjiang, Guangdong 524088, China

Received:2022-09-08 Online:2023-03-20 Published:2023-03-14
Contact: *

亚精胺对盐胁迫下黄华占水稻幼苗根系抗氧化酶活性及Na⁺稳态的影响

黄婷婷¹^,², 郑殿峰¹^,², 冯乃杰¹^,², 赵黎明¹^,², 周鸿凯¹^,², 沈雪峰¹^,²

1.广东海洋大学滨海农业学院，广东湛江 524088
2.国家耐盐碱水稻技术创新中心华南中心，广东湛江 524088

通讯作者: *
基金资助:
广东海洋大学国家级创新创业训练计划项目(CXXL2020013);广东海洋大学科研启动费资助项目(R20062);广东省重点领域研发计划项目(2020B020219004)

Abstract

Abstract:

The effects of exogenous spermidine （Spd） on rice seedling growth, antioxidant enzyme activity, reactive oxygen species （ROS） metabolism, malonic dialdehyde （MDA） and Na⁺ homeostasis were studied with rice variety Huanghuazhan as the material. The results showed that the content of ROS、·O₂^-、H₂O₂、Na⁺ and MDA were significantly increased in the root of rice seedlings by treated with 100 mmol·L^-1 NaCl, meanwhile, the activities of catalase （CAT） and peroxidase （POD） were remarkably reduced. However, the activities of CAT and POD were significantly enhanced by using 0.1 mmol·L^-1 Spd, and the content of ROS、·O₂^-、H₂O₂、Na⁺ and MDA were remarkably decreased. So, the damage of Na⁺ toxicity and membrane oxidation were alleviated, the stability of biofilm system was protected, and the steady state of cells was maintained with Spd under salt stress condition.

Key words: rice, exogenous spermidine, salt stress, antioxidant defense, Na⁺

摘要：

以黄华占种子为材料，研究盐胁迫条件下，外源亚精胺（Spd）对水稻幼苗根系的生长、抗氧化酶活性、活性氧（ROS）代谢、丙二醛（MDA）以及Na⁺稳态的影响。结果表明，经100 mmol/L NaCl溶液处理水稻幼苗根系的ROS、·O₂^-、H₂O₂、Na⁺和MDA含量显著升高，过氧化物酶（POD）和过氧化氢酶（CAT）活性显著降低；在盐胁迫条件下，施用0.1 mmol/L Spd显著提高水稻幼苗根系的CAT和POD活性，显著降低ROS、·O₂^-、H₂O₂、Na⁺和MDA含量，从而减轻Na⁺毒害和过氧化造成的膜损伤，保护生物膜系统的稳定，维持细胞的稳态。

关键词: 水稻, 外源Spd, 盐胁迫, 抗氧化防御, Na⁺

CLC Number:

S511

HUANG Tingting, ZHENG Dianfeng, FENG Naijie, ZHAO Liming, ZHOU Hongkai, SHEN Xuefeng. Effects of Spermidine on Antioxidant Enzyme Activity and Na⁺ Homeostasis of Seedlings Roots of Huanghuazhan Rice under Salt Stress[J]. China Rice, 2023, 29(2): 43-47.

黄婷婷, 郑殿峰, 冯乃杰, 赵黎明, 周鸿凯, 沈雪峰. 亚精胺对盐胁迫下黄华占水稻幼苗根系抗氧化酶活性及Na⁺稳态的影响[J]. 中国稻米, 2023, 29(2): 43-47.

Figures/Tables 7

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