不同复合种养模式稻田土壤细菌群落结构比较分析

doi:10.3969/j.issn.1006-8082.2022.03.008

摘要/Abstract

摘要：

利用高通量测序技术，在Illumina系统上对16 srRNA基因的V3~V4区进行测序，比较分析了“河蟹-稻”“非洲雁-稻”和“龙虾-稻”三种复合种养模式结穗期时稻田土壤细菌群落结构、多样性和丰度。各样本共检测到3 101个OTU，可将细菌分为48门、115纲、223目、39科、71属、1335种。三种复合种养模式的优势菌门相近，但有各自独有的菌属。Venn图分析表明，龙虾稻田物种数目最多，而非洲雁稻田独有物种数目最多。样本层级聚类分析结果表明，河蟹稻田和龙虾稻田的细菌群落结构相近，而与非洲雁稻田有较大差异。河蟹稻田的多样性指数Shannon值、丰富度指数Ace值和Chao值均高于龙虾稻田和非洲雁稻田，说明河蟹稻田有更好的土壤细菌生态组成。

关键词: 水稻, 高通量测序, 群落结构, 河蟹稻田, 非洲雁稻田, 龙虾稻田

Abstract:

The structure, diversity and abundance of bacterial community in soil of crab paddy field, African goose paddy field and lobster paddy field at heading stage were compared and analyzed by sequencing the V3-V4 region of 16srRNA gene in Illumina system with high-throughput sequencing technology. A total of 3 101 OUTs were detected in each sample, which could be divided into 48 phyla, 115 classes, 223 orders, 39 families, 71 genera and 1 335 species. The three complex cultivation models had similar dominant microflora, but each had its own unique genus. Venn diagram analysis showed that the number of species in lobster paddy field was the largest, and the number of unique species in African goose paddy field was the largest. The results of hierarchical cluster analysis showed that the bacterial community structure of crab paddy field and lobster rice field was similar, but different from that of African goose rice field. The Shannon value, ACE value and Chao value of diversity index, richness index of crab paddy field were higher than those of lobster paddy field and African goose paddy field, which indicated that crab paddy field had better soil bacterial ecological composition.

Key words: rice, high-throughput sequencing, community structure, crab paddy field, African goose paddy field, lobster paddy field

中图分类号:

S511.061

宋宇, 丛培东, 曲贵伟, 杨清海. 不同复合种养模式稻田土壤细菌群落结构比较分析[J]. 中国稻米, 2022, 28(3): 47-51.

SONG Yu, CONG Peidong, QU Guiwei, YANG Qinghai. Comparative Analysis of Bacterial Community Structure in Paddy Soil Under Different Complex Cultivation Models[J]. China Rice, 2022, 28(3): 47-51.

图/表 6

表1 16SrRNA基因文库97%相似性的OTU丰富度和多样性指数比较

图1 样品稀释曲线

图2 物种Venn分析图

图3 稻田土壤细菌群落距离Heatmap图

图4 三种模式下稻田土壤门水平的细菌组成丰度图相对丰度低于0.01%的物种归于“其他”组内。

表2 三种模式下稻田土壤不同属的分布

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