利用高密度遗传图谱定位两种不同环境的水稻剑叶形态性状QTL差异

doi:10.3969/j.issn.1006-8082.2024.06.005

中国稻米 ›› 2024, Vol. 30 ›› Issue (6): 29-34.DOI: 10.3969/j.issn.1006-8082.2024.06.005

利用高密度遗传图谱定位两种不同环境的水稻剑叶形态性状QTL差异

王兴宇¹(), 王静¹, 徐群², 章孟臣², 王珊², 孙燕飞², 魏兴华², 杨窑龙², 郭晓红¹, 冯跃¹^,²^,^*()

¹黑龙江八一农垦大学农学院，黑龙江大庆 163319
²中国水稻研究所/国家水稻改良中心/水稻生物育种全国重点实验室，杭州 310006

收稿日期:2023-11-30 出版日期:2024-11-20 发布日期:2024-11-19
通讯作者: ^*fy_555500@163.com
作者简介:wxy13359613181@163.com
基金资助:
黑龙江省哲学社会科学规划项目“基于新发展理念的黑龙江省农业高质量发展时空演化及提升路径研究”(21JYE400)

The Difference of QTL for Morphological Traits of Rice Flag Leaf by High-density Genetic Map in Two Different Environments

WANG Xingyu¹(), WANG Jing¹, XU Qun², ZHANG Mengchen², WANG Shan², SUN Yanfei², WEI Xinghua², YANG Yaolong², GUO Xiaohong¹, FENG Yue¹^,²^,^*()

¹College of Agricultural Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China
²China National Rice Research Institute/China National Center for Rice Improvement/ State Key Laboratory of Rice Biology and Breeding, Hangzhou 310006, China

Received:2023-11-30 Published:2024-11-20 Online:2024-11-19
Contact: ^*fy_555500@163.com
About author:wxy13359613181@163.com

摘要/Abstract

摘要：

水稻剑叶形态性状是构成理想株型的重要因素之一，发掘新的控制剑叶形态性状的基因资源是提高水稻产量的重要途经。本研究利用高密度遗传图谱对在杭州和海南陵水种植的印度粳稻品种M494和中国籼稻品种中9B（Z9B）杂交衍生的重组自交系（recombinant inbred lines，RIL）群体剑叶长、剑叶宽、剑叶面积和剑叶长宽比进行数量性状位点（Quantitative trait locus, QTL）定位检测。研究共检测到10个QTL，包括1个剑叶长QTL、4个剑叶宽QTL、4个剑叶面积QTL和1个剑叶长宽比QTL，分布于第4、第7、第8、第9和第10号染色体，贡献率介于6.47%~13.28%之间；鉴定到2个多效性QTL区间，其中在第4号染色体Block536~Block544区间检测到同时控制剑叶宽和剑叶面积的QTL，在第10号染色体Block942~Block961区间检测到同时控制剑叶宽和剑叶长宽比的QTL，其贡献率分别为8.82%、13.28%、12.28%和12.60%。两地间QTL差异较大，仅在Block536~Block544区间同时定位到控制剑叶宽及剑叶面积的QTL及在Block942~Block961区间同时定位到控制剑叶宽及剑叶长宽比的QTL。

关键词: 水稻, 剑叶形态, 重组自交系, 高密度图谱, QTL定位

Abstract:

The flag leaf morphological traits are one of the important factors that constitute the ideal plant type in rice. Exploring new genetic resources that control the flag leaf morphological traits is one of the important ways to increase rice yield. In this study, quantitative trait loci (QTLs) were detected for flag leaf length (FLL), flag leaf width (FLW), flag leaf area (FLA), and flag leaf length to width (FLLW) of a population of recombinant inbred lines (RIL) derived from a cross between the Indian japonica rice variety M494 and China indica variety Zhong 9B (Z9B) planted at Hangzhou and Hainan using high-density genetic map. A total of 10 QTL were detected including one QTL for flag leaf length, four QTL for flag leaf width, four QTL for flag leaf area and one QTL for flag leaf aspect ratio, distributed on chromosomes 4, 7, 8, 9 and 10, which could explain 6.47%-13.28% of the phenotypic variation. Two pleiotropic QTL intervals were identified, and the locus was located between Block536 and Block544 on chromosome 4 which were simultaneously controlled FLW and FLA, and the locus between Block942 and Block961 on chromosome 10 simultaneously controlling FLW and FLLW, with phenotypic variation were 8.82%, 13.28%, 12.28% and 12.60%, respectively. The QTL varied greatly between the two sites, with QTL controlling flag leaf width and flag leaf area only simultaneously position in Block536-Block544 and QTL controlling flag leaf width and flag leaf length to width only in the Block942-Block961 interval.

Key words: rice, flag leaf morphology, RIL, high-density genetic map, QTL mapping

中图分类号:

S511

王兴宇, 王静, 徐群, 章孟臣, 王珊, 孙燕飞, 魏兴华, 杨窑龙, 郭晓红, 冯跃. 利用高密度遗传图谱定位两种不同环境的水稻剑叶形态性状QTL差异[J]. 中国稻米, 2024, 30(6): 29-34.

WANG Xingyu, WANG Jing, XU Qun, ZHANG Mengchen, WANG Shan, SUN Yanfei, WEI Xinghua, YANG Yaolong, GUO Xiaohong, FENG Yue. The Difference of QTL for Morphological Traits of Rice Flag Leaf by High-density Genetic Map in Two Different Environments[J]. China Rice, 2024, 30(6): 29-34.

图/表 7

表1 水稻RIL群体遗传连锁图染色体标记分布

表2 亲本、RIL群体剑叶形态性状在富阳及陵水的表型值

图1 富阳和陵水RIL群体剑叶形态性状表型分布频率

表3 RIL群体剑叶形态性状的相关系数

图2 M494/Z9B重组自交系群体的遗传图谱

表4 水稻剑叶形态性状的QTL分析

图3 水稻剑叶形态性状QTL在染色体上的分布

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利用高密度遗传图谱定位两种不同环境的水稻剑叶形态性状QTL差异

The Difference of QTL for Morphological Traits of Rice Flag Leaf by High-density Genetic Map in Two Different Environments

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